嵌合异硫氰酸罗丹明B核壳荧光纳米颗粒制备的新方法研究  

New Method for Preparing Core-shell Fluorescent Nanoparticles Doped with Rhodamine B Isothiocyanate

作　　者：原茵[1] 何晓晓[1] 王柯敏[1] 谭蔚弘[1] 彭娇凤[1] 黄杉生[1] 林霞[1] 

机构地区：[1]湖南大学化学化工学院,化学生物传感与计量学国家重点实验室,湖南省生物纳米工程技术研究中心,长沙410082

出　　处：《高等学校化学学报》2005年第3期446-448,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金重点项目 (批准号 :2 0 13 5 0 10 );国家重点基础研究发展规划项目 (批准号 :2 0 0 2 CB5 13 10 0 -10 );教育部科学技术研究项目 (批准号 :0 3 181);国家科技计划项目 (批准号 :2 0 0 3 BA 3 10 A16);国家自然科学基金 (批准号 :2 0 40 5 0 0 5

摘　　要：In this article, the preparation of core-shell fluorescent dye nanoparticles doped with rhodamine B isothiocyanate by using novel method is reported, which involves synchronous hydrolysis of tetraethoxysilane(TEOS) in water in oil microemulsion with the combination of N-(β-amimoethyl)-γ-amino propyltriethoxy-silane(AEAPS) and rhodamine B isothiocyanate(TRITC). This method conquers the disadvantages of traditional preparation method about organic dye doped core-shell nanoparticles such as the leakage of fluorescent dyes and the low encapsulation efficiency of fluorescent dyes. The fluorescent nanoparticle prepared by using the novel method have the advantages such as high fluorescence and good fluorescent stability, which are hopeful to be applied in the field of biological labelling.In this article, the preparation of core-shell fluorescent dye nanoparticles doped with rhodamine B isothiocyanate by using novel method is reported, which involves synchronous hydrolysis of tetraethoxysilane(TEOS) in water in oil microemulsion with the combination of N-(β-amimoethyl)-γ-amino propyltriethoxy-silane(AEAPS) and rhodamine B isothiocyanate(TRITC). This method conquers the disadvantages of traditional preparation method about organic dye doped core-shell nanoparticles such as the leakage of fluorescent dyes and the low encapsulation efficiency of fluorescent dyes. The fluorescent nanoparticle prepared by using the novel method have the advantages such as high fluorescence and good fluorescent stability, which are hopeful to be applied in the field of biological labelling.

关 键 词：异硫氰酸罗丹明B(TRITC) 油包水微乳液 同步水解 核壳荧光纳米颗粒 

分 类 号：O657[理学—分析化学]