肉苁蓉茎水溶性多糖SPA的结构分析  

Structural Analysis of Water-soluble Polysaccharide SPA Isolated from the Stem of the Cistanche Deserticola Ma

作　　者：赵伟[1] 颜宏[1] 梁忠岩[1] 张雅君[1] 焦昕倩[2] 

机构地区：[1]东北师范大学生命科学学院 [2]吉林大学环境与资源学院,长春130025

出　　处：《高等学校化学学报》2005年第3期461-463,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :3 9870 847)资助 .

摘　　要：A water-soluble crude polysaccharide was isolated from the stem of the wild Cistanche deserticola Ma. It was deproteinized by Sevage method and proteinase method, and fractionated by ultrafiltration and Sephadex G-75 column chromatography. A polysaccharide fraction SPA was collected. The polysaccharide of SPA consisted of Rha, Ara, Man, Gal and Glc in the molar ratio of 1∶1.83∶3.06∶4.56∶14.74, and the average molecular weight of SPA was around 0.76×104. By means of partial hydrolysis, Smith degradation, mathylation analysis, GC-MSIR and 13C NMR, the results revealed the main chain was made up of (1→6)-linked-Glc residues and substituted at 3-O. On an average, there was one branch among seven main chain residues. The side chains is composed of (1→4)-linked-Gal, (1→4)-linked-Man, (1→3)-linked-Gal and (1→2)-linked-Rha residues. The nonreduced end is composed of (1→)Ara.A water-soluble crude polysaccharide was isolated from the stem of the wild Cistanche deserticola Ma. It was deproteinized by Sevage method and proteinase method, and fractionated by ultrafiltration and Sephadex G-75 column chromatography. A polysaccharide fraction SPA was collected. The polysaccharide of SPA consisted of Rha, Ara, Man, Gal and Glc in the molar ratio of 1∶1.83∶3.06∶4.56∶14.74, and the average molecular weight of SPA was around 0.76×104. By means of partial hydrolysis, Smith degradation, mathylation analysis, GC-MSIR and 13C NMR, the results revealed the main chain was made up of (1→6)-linked-Glc residues and substituted at 3-O. On an average, there was one branch among seven main chain residues. The side chains is composed of (1→4)-linked-Gal, (1→4)-linked-Man, (1→3)-linked-Gal and (1→2)-linked-Rha residues. The nonreduced end is composed of (1→)Ara.

关 键 词：肉苁蓉 多糖 结构分析 

分 类 号：O629[理学—有机化学]