山东省HIV-1感染者分子流行病学研究  

Study on the molecular epidemiology of people infected with human immunodeficiency virus-1 in Shandong province

作　　者：傅继华[1] 李盛长 张静[1] 刘学真[1] 黄涛[1] 邢辉[3] 邵一鸣 

机构地区：[1]山东省疾病预防控制中心艾滋病防治所,济南250014 [2]济南市长清区中医医院 [3]中国疾病预防控制中心性病艾滋病预防控制中心

出　　处：《中华流行病学杂志》2005年第2期124-127,共4页Chinese Journal of Epidemiology

摘　　要：目的　了解流行于山东省境内艾滋病病毒 (HIV 1)毒株的亚型及各种亚型的分布特点。方法　对 93例HIV感染者进行详细的流行病学调查 ,并采集外周静脉防凝血 ,提取前病毒DNA进行体外扩增 ,获得包膜蛋白 (env)、核心蛋白 (gag)、tat区基因的核酸片段 ,并对各基因区核苷酸进行测定和分析。结果　发现在所有目标人群中共存在B、B’、C 3种亚型以及CRF0 7 BC、CRF0 8 BC、CRF0 2 AG和CRF0 1 AE 4种重组毒株 ,B’亚型在人群和地域上分布最广 ,涉及献血员、受血者、二者的配偶和性乱人群 ,分布在山东省 10个地市 ;CRF0 7 BC、CRF0 8 BC亚型以吸毒人群为主 ,分布在 5个地市 ;CRF0 1 AE和其他亚型则主要为性乱人群 ,集中在几个经济发达的城市。结论　山东省HIV 1亚型众多 ,各亚型在我省高危人群和地域分布不平衡 ,各有特点 ,从总的亚型流行特征来看 ,HIV 1在我省有流行加快的趋势。Objective To study the distribution of human immunodeficiency virus-1(HIV-1) strains subtypes in Shandong province and to study their source in order to predict the epidemic trend. Methods Epidemiological investigation was made and 93 DNA fragments of HIV-1 env,gag,tat gene were amplified by nested polymerase chain reaction from people infected with HIV-1, in 2002-2003. Their C2-V3, P17/P24, 1st exon of tat and adjacent region were sequenced. Results Sequence analysis showed that there were 7 HIV-1 strains or circulating recombinant forms(CRFs), B’( n = 71), CRF01-AE( n = 9),CRF07-BC( n = 3), CRF08-BC( n = 3), B( n = 2),C( n = 2), CRF02-AG( n = 2). B’ strains was the predominant which, covered 10 cities and 4 kinds of population including blood donors, blood receivers, spouses of the infected people and clients of the sex workers. CRF07-BC、CRF08-BC strains were identified in 5 cities, mainly from injecting drug users. CRF01-AE and other strains were found distributed in developed cities, among sex workers. Conclusion There were many kinds of subtypes and CRFs of HIV and their genomes which generated obvious variation in Shandong province, suggesting that they might facilitate the spread of the disease in Shandong province.

关 键 词：亚型 CRF HIV-1感染 性乱人群 分子流行病学 基因 包膜蛋白 山东 结论 发达 

分 类 号：R512.91[医药卫生—内科学] R692.5[医药卫生—临床医学]