佛波脂对体外培养Th细胞表面CD4分子表达的影响  

Effects of PMA on CD4 expression on T-helper surface in vitro

作　　者：叶卫东[1] 张循善[2] 孙小梅[3] 连莉[4] 徐建华[4] 李庆[3] 

机构地区：[1]武警安徽总队医院检验科,合肥230041 [2]安徽医科大学第一附属医院输血科,合肥230032 [3]安徽省立医院中心实验室,合肥230001 [4]安徽医科大学第一附属医院风湿科,合肥230032

出　　处：《免疫学杂志》2005年第2期104-106,共3页Immunological Journal

基　　金：安徽省教育厅自然科学基金资助项目 (2 0 0 1kj14 7)

摘　　要：目的　探索佛波脂(PMA)对Th细胞表面CD4分子表达的影响。方法　用荧光标记的抗CD3 4 8抗体同经10、15、2 0、2 5ng mL的PMA和1μg mL的Ionomycin刺激培养2、3、4h的正常人外周全血细胞中的Th细胞上的CD4分子结合,溶血后用流式细胞仪检测CD4通道的荧光强度(MFI)。结果　CD4通道的MFI随PMA质量浓度的升高和刺激时间的延长而降低。结论　Th细胞表面CD4分子在刺激培养过程中随PMA质量浓度的升高和刺激时间的延长而减少。Objective To investigate the effects of PMA (Phorbol 12-myristate 13-Acetate) on CD4 expression on T-helper surface. Methods Peripheral whole-blood of healthy donors were co-cultured with 10, 15, 20, 25 ng/mL PMA and Ionomycin (1 μg/mL) for 2, 3, and 4 h respectively, then fluorescein-conjugated antibodies of CD3/4/8 were added. The mean fluorescent intensity (MFI) of CD4 channel was measured by flow-cytometry. Results The MFI of CD4 channel decreased with the increase of PMA concentration and the prolonging of co-culture time. Conclusion The surface CD4 of T-helper is decreased with the concentration of PMA increased and the co-cultured hours prolonged.

关 键 词：佛波脂 CD4 平均荧光强度 

分 类 号：R446.6[医药卫生—诊断学]