内耳免疫反应中细胞凋亡及Caspase-3表达的研究  

A Study on Apoptosis and Caspase-3 Expression in the Immune Response of Inner Ear

作　　者：许丽娟[1] 龚树生[2] 汪吉宝[2] 黄翔[2] 

机构地区：[1]武汉科技大学医学院五官科,湖北武汉430080 [2]华中科技大学同济医学院附属协和医院耳鼻咽喉科

出　　处：《中国医师杂志》2005年第2期178-180,共3页Journal of Chinese Physician

基　　金：国家自然科学基金资助项目 (30 0 70 81 0 ) ;教育部"第四届高校青年教师奖"资助项目

摘　　要：目的　研究内耳免疫反应过程中是否存在细胞凋亡 ,以及细胞凋亡是否与Caspase -3信号转导有关。方法　选用雌性白色豚鼠 16只 ,随机分为实验组和对照组各 8只 ,以钥孔虫戚血蓝蛋白 (keyholelimpethemocyanin ,KLH)全身免疫后 ,实验组以相同抗原进行内耳免疫 ,对照组内耳注射等量的磷酸盐缓冲生理盐水 (PBS) ,在内耳免疫 5d后处死动物 ,取内耳免疫侧耳蜗做石蜡切片。通过TUNEL法检测内耳凋亡细胞 ,免疫组化检测内耳Caspase -3的表达。结果　实验组Corti器 ,血管纹、螺旋韧带和螺旋神经节存在TUNEL染色阳性细胞 ,而对照组仅在支持细胞、血管纹和螺旋神经节中发现极少数TUNEL染色阳性细胞。免疫组化染色实验组Caspase -3表达阳性 ,而对照组表达阴性。结论　内耳免疫反应可以诱导细胞凋亡的发生 ;内耳免疫反应诱导细胞凋亡的发生中有Caspase -3的激活。Objective To investigate whether apoptosis is invovled in the immune response of inner ear,and relation between the signal transduction of Caspase-3 and apoptosis. Methods Sixteen healthy,female guinea pigs were employed in this study. The animals in the experiment group were sensitized systematically with keyhole limpet hemocyanin(KLH),then were inoculated in inner ear with the same antigen, and the animals in the control group were injected with the same volume of PBS. The animals were sacrificed at the 5th day after inner ear vaccination. Paraffin sections of cochleas from animals were prepared, TUNEL assay was used to detect apoptotic cells in inner ears, and immunohistochemical method was applied to detect the expression of Caspase-3 in inner ears. Results TUNEL-positive cells are found in the inner ears of the experiment group, but did not in the inner ears of the control group except for a few TUNEL-positive cells in the supporting cells, the stria vascular cells and the spiral ganglion cells. The high expression of Caspase-3 could be detected in the inner ears of the experiment group, but no positive cells of Caspase-3 were found in the inner ears of the control group. Conclusion Apoptosis could be induced by the immune response of inner ear, and Caspase-3 cascade may be involved in the course of apoptosis.

关 键 词：内耳 CASPASE-3 免疫反应 对照组 细胞凋亡 表达 TUNEL 血蓝蛋白 雌性 支持细胞 

分 类 号：R764[医药卫生—耳鼻咽喉科] R735[医药卫生—临床医学]