结核分支杆菌培养上清诱导呼吸道上皮细胞IL-8表达  

Production of IL-8 in airway epithelial cells induced by Mycobacterium tuberculosis supernatant

作　　者：王芳[1] 冯艳[1] 陈襄文[1] 冯云[1] 黄宁[1] 王伯瑶[1] 吴琦[1] 

机构地区：[1]四川大学华西医学中心感染免疫研究室,成都610041

出　　处：《四川生理科学杂志》2004年第1期10-13,共4页Sichuan Journal of Physiological Sciences

基　　金：国家自然科学基金 (No .30 2 70 6 88);CMB基金(No.98 86 1)资助

摘　　要：目的 :观察呼吸道上皮细胞对结核杆菌产物产生的炎症反应 ,探讨炎症反应信号传递的机制。方法 :制备结核分枝杆菌强毒力H37RV株培养上清 ,用培养上清刺激肺上皮细胞株SPC A 1和A5 49,酶联免疫吸附实验检测细胞IL 8表达量。用突变MyD88表达重组质粒转染SPC A 1细胞 ,观察MyD88与结核杆菌培养上清诱导上皮细胞炎症反应信号传递的关系。结果 :结核分枝杆菌强毒力H37RV株培养上清明显增加SPC A 1和A5 49细胞IL 8的表达。用突变MyD88表达重组质粒 pcDNA3 .1 mMyD88转染SPC A 1 ,结果显著抑制了结核杆菌培养上清对SPC A 1细胞的炎症刺激作用。结论 :结核杆菌培养上清能够诱导呼吸道上皮细胞IL 8表达 ,Toll/IL 1受体信号通路及MyD88分子与细胞炎症反应的信号传递相关 ,提示在肺结核病发病过程中 ,结核杆菌的代谢产物可能刺激肺上皮细胞产生炎症细胞因子 。Objective:This study was done to investigate the inflammatory response of airway epithelial cells to the components in Mycobacterium tuberculosis supernatant and the mechanisms of the inflammatory signaling transduction. Methods: Mycobacterium tuberculosis supernatant was prepared from virulent laboratory strain H 37 RV. Human lung epithelial cell lines SPC A 1 and A549 were challenged with the supernatant and IL 8 secretion by the epithelial cells was assayed by using ELISA. In order to investigate whether MyD88 is involved in the inflammatory signaling pathway SPC A 1 cells were transfected with an expression plasmid encoding dominant negative mutant MyD88. Results: Mycobacterium tuberculosis supernatant from H 37 RV elicited production of IL 8 by SPC A 1 and A549 cell lines. Expression of dominant negative mutant MyD88 in the SPC A 1 cell line effectively blocked the production of IL 8 in response to Mycobacterium tuberculosis supernatant. Conclusion:Components in Mycobacterium tuberculosis supernatant could induce IL 8 expression in airway epithelial cells via Toll/IL 1 receptor signal transduction pathway. The production of inflammatory cytokines by the airway epithelial cells in response to Mycobacterium tuberculosis and/or its products may contribute to pathogenesis of pulmonary tuberculosis.

关 键 词：呼吸道上皮细胞 培养上清 IL-8 结核分支杆菌 SPC-A-1细胞 结核分枝杆菌 结核杆菌 H37RV 炎症反应 信号传递 酶联免疫吸附 A549细胞 pcDNA3 受体信号通路 炎症细胞因子 重组质粒 上皮细胞株 肺上皮细胞 实验检测 刺激作用 

分 类 号：R392[医药卫生—免疫学]