大鼠糖尿病早期肾脏皮质Na^+,K^+-ATP酶α_1-亚单位的表达  

Na^+,K^+-ATPase expression of α_1-subunit in renal cortex of early diabetic rats

作　　者：赵焕新[1] 高原[1] 

机构地区：[1]遵义医学院生理学教研室,遵义563003

出　　处：《四川生理科学杂志》2004年第2期58-60,共3页Sichuan Journal of Physiological Sciences

摘　　要：目的 :探讨大鼠糖尿病早期肾脏肥大和肾脏皮质Na+ ,K+ ATP酶α1 亚单位mRNA表达的变化。方法 :采用单剂量腹腔注射链脲佐菌素 (streptozocozin ,STZ)诱发大鼠糖尿病 ,分别在糖尿病第 1、3、7天测定肾脏肥大指数 ,并以RT PCR技术检测肾脏皮质Na+ ,K+ ATP酶α1 亚单位mRNA的表达。结果 :大鼠糖尿病早期血糖显著升高 ,肾脏肥大 ,肾脏皮质Na+ ,K+ ATP酶α1 亚单位mRNA的表达明显降低。结论 :肾脏皮质Na+ ,K+ ATP酶α1 亚单位mRNA表达的改变可能是糖尿病早期肾脏肥大的一个因素。Objective: To explore the renal hypertrophy and the alteration of Na+,K+-ATPase expression of α 1-subunit mRNA in diabetic rats’ renal cortex. Methods: Diabetic rats were induced by intraperitoneal injection of STZ. The renal hypertrophy was detected by K.W./100g B.W; Na+,K+-ATPase expression of α 1-subunit mRNA in renal cortex was measured by RT-PCR on the 1st, 3th, 7th day after diabetes mellitus. Results: The level of blood glucose and K.W./100g B.W significantly increased in diabetic rats. Na+,K+-ATPase expression of α 1-subunit mRNA in renal cortex of diabetic rats decreased. Conclusion: The decrease of Na+,K+-ATPase expression of α 1-subunit mRNA in renal cortex can be one factor for renal hypertrophy of early diabetes mellitus.

关 键 词：K^+-ATP酶 Na^+ 糖尿病 亚单位 皮质 早期 大鼠 mRNA表达 PCR技术检测 肾脏肥大 链脲佐菌素 腹腔注射 单剂量 RT- 

分 类 号：R587.1[医药卫生—内分泌]