人脐血CD34^+细胞体外Dexter基质细胞培养后细胞表面分子变化的动态观察  

Observation on the cell surface molecule of DEXTER culture of cord CD34^+ cells in vitro

作　　者：高蕾[1] 张曦[1] 陈幸华[1] 刘林[1] 彭贤贵[1] 孔佩艳[1] 刘红[1] 张怡[1] 王庆余[1] 

机构地区：[1]第三军医大学新桥医院血液科,重庆400037

出　　处：《重庆医学》2005年第3期392-394,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(30070327);第三军医大学科研基金资助项目(XG200347)

摘　　要：目的　动态观察人脐血CD34+细胞Dexter基质细胞培养后细胞表面分子的变化情况。方法　应用 MACS磁珠分离系统分离脐带血CD34+细胞,按照Dexter法行脐血基质细胞培养,观察不同时间基质细胞的生长状态及细胞表面分子的变化情况。结果　脐血基质细胞在生长情况、细胞形态与骨髓基质细胞有不同之处,但其细胞表面分子的表达特点却与骨髓基质细胞有类似之处。结论　脐血CD34+细胞在生长因子的辅助下能促进基质细胞集落的形成,有望成为新的基质细胞来源。Objective To observe contineously on the cell surface molecule of Dexter culture of cord CD34 + cells in vitro.Methods CD34 + cord cells were separated by MACS system from 8 fresh samples.The methods of Dexter culture system of stromal cells were used to observe the change of cell surface molecule of cord stromal cell on the different time.Results Cord stromal cells are different from bone marrow stromal cells at many aspects such as growth condition, conformation, cell morphology etc, but they are similar to bone marrow stromal cells at the expression of cell surface molecule.Conclusion The results indicate that some hematopoietic growth factors also have promoting effects on the expansion of cord stromal cells,and which may be become a new resource of stromal cells.

关 键 词：基质细胞 CD34^+细胞 免疫组织化学 细胞化学 细胞表面分子 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]