库尔勒香梨总RNA的快速提取方法  

A Method of Rapidly Extracting Total RNA from Kurle Aroma Pear

作　　者：何梅[1] 李海生[1] 赵英[1] 牛建新[1] 马兵刚[1] 

机构地区：[1]石河子大学农学院园艺系,新疆石河子832003

出　　处：《石河子大学学报（自然科学版）》2004年第6期474-476,共3页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金资助项目(30060053);(30360066);新疆生产建设兵团科委项目(NKB02SDXNK01SW);国家科技攻关计划引导项目(2003BA546C)

摘　　要：以库尔勒香梨花、芽、茎尖、叶片和皮层为材料,对总RNA提取方法进行了改进,从而筛选出适合库尔勒香梨总RNA的提取方法。采用该方法可以在3～4h内得到纯度高、含量高、完整性好的梨总RNA,并获得了逆转录活性较强的病毒RNA,同时使提取成本降低。此方法对苹果、葡萄、桃等果树总RNA的提取均适用。Using Kurle aroma pear′s leaves,flowers,shoot tips,phloem and buds as materials,the paper describes the procedure of extraction of RNA,and the fittest methods for Kurle roma pear were screened out.The results showed that the reverse-transcritable RNA can be obtained in 3～4 hours.The cost of RNA extraction by this method is much lower than any other method.This method is suitable for total RNA extraction in apple,peach,grapevine ect..

关 键 词：库尔勒香梨 总RNA 茎尖 果树 葡萄 苹果 快速提取方法 皮层 逆转录 含量 

分 类 号：S661[农业科学—果树学] S436[农业科学—园艺学]