RAPD标记对喀斯特高海拔山区适宜玉米自交系遗传多样性的研究  

Study on Genetic Diversity of Maize Inbred Lines in Karst High Elevation Mountain Area by RAPD Markers

作　　者：彭忠华[1] 张明生[2,3] 高翔[4] 徐如宏[1] 王进华[1] 顾金春[1] 戴保威[1] 

机构地区：[1]贵州大学农学院 [2]南京大学生命科学学院 [3]贵州大学农学院,贵州贵阳550025 [4]贵州省农业科学院

出　　处：《玉米科学》2005年第1期10-14,共5页Journal of Maize Sciences

基　　金：贵州省"十五"重大攻关项目[黔科合农社字2001(1104号)];贵州大学校基金项目赞助

摘　　要：利用RAPD分子标记技术研究了我国喀斯特高海拔山区适宜玉米自交系的遗传多样性,用23条引物对我国喀斯特高海拔山区适宜玉米自交系进行遗传多样性研究和杂种优势群划分。从90个随机引物中筛选出23个多态性好的引物扩增材料,产生出201条多态带,多态性位点比率为81.6%,通过UPGMA聚类分析,将我国喀斯特高海拔山区玉米地方自交系划分为7个类群。RAPD markers technique has been used to study the diversity of maize suitable inbred lines in Karst high elevation mountainous area. The screening of the 90 random primers allowed the selection of 23 polymorphic primer used for the analysis. A total of 201 polymorphic bands were produced from the 23 selected primers. Through clustering on RAPD markers by UPGMA(Unweighed Pair-Group Method Using Arithmetic Average), Mean polymorphic information content was 0.651. Clustering analysis was conducted basing on the genetic similarity coefficient. The maize suitable inbreds were classified into seven groups.

关 键 词：玉米自交系 RAPD 遗传多样性 喀斯特高海拔山区 

分 类 号：S513.024[农业科学—作物学]