恩诺沙星对鲤酯酶同工酶和血清生理生化指标的影响  

Effects of Enrofloxacin on hematological values and esterase in carp

作　　者：巩华[1] 李华[1] 于毅[1] 曲翊[1] 王恺[1] 

机构地区：[1]大连水产学院生命科学与技术学院,辽宁大连116023

出　　处：《大连水产学院学报》2005年第1期29-33,共5页Journal of Dalian Fisheries University

基　　金：辽宁省自然科学基金资助项目 (20032097)

摘　　要：采用聚丙烯酰胺凝胶电泳和试剂盒等方法, 对投喂不同剂量恩诺沙星和氯霉素的鲤Cyprinuscarpio酯酶同工酶进行了电泳分析, 同时对一些血清生理生化指标进行了测定。结果表明: 恩诺沙星可使鲤酯酶同工酶的活性升高; 所有投喂药饵的鱼血清总蛋白、白蛋白、白蛋白与球蛋白之比均下降, 谷丙转氨酶、碱性磷酸酶、无机磷、非蛋白氮、肌酐呈上升趋势; 高浓度药物对鲤的器官生理功能均有不同程度的影响, 其中对肝胰脏、肾脏的影响较为明显。Esterase (EST) isozymes and some hematological values were determined in live, kidney, muscle, intestine, gill and spleen of cultured common carp (Cyprinus carpio) to evaluate the effects of different dosages of enrofloxacin (ERFX) and chloramphenicol (CAP) by polyacylamide electrophoresis (PAGE). The results showed that the carp fed the diet containing ERFX had significantly high EST activities. There were significantly low serum total protein (TP),albumin(A) and the rate of A/G in the fish fed the diet containing ERFX and chloramphenicol (CAP). However, while there were significantly high serum levels of glutamic-pyruvic transaminase (GPT), alkaline phosphatase (ALP), inorganic phosphorus (IOP), non-protein nitrogen (NPN) and carnine (CR) in the fish fed the diets containing the drugs, indicating that the drugs influenced all the organs and tissues on some extent, significantly the liver and kidney.

关 键 词：恩诺沙星 鲤 酯酶同工酶 血清指标 

分 类 号：Q599[生物学—生物化学]