乙型肝炎患者外周血IL-18与其他细胞因子水平的相关性研究  

The clinical significance of serum IL-18 level in the peripheral blood of HBV-infected patients

作　　者：江华[1] 程正江[1] 张海蓉[1] 孙伯良[1] 曾平凡[1] 曹红[1] 

机构地区：[1]湖北省襄樊市中心医院检验科,441021

出　　处：《国外医学（临床生物化学与检验学分册）》2005年第2期69-71,共3页Foreign Medical Sciences(section of Clinical Biochemistry and Laboratory Medicine

摘　　要：　目的　探讨乙型肝炎病毒(HBV)感染患者外周血白细胞介素-18(IL -18)与其他细胞因子水平的关系,并研究血清中IL -18的表达水平与HBV感染的相关性及其临床意义。方法　用微粒子酶免法测得乙型肝炎患者与常规体检乙肝三系结果后将其分为3 组:HBsAg、HBeAg、HBcAb阳性组(大三阳组),HBsAg、HBeAb、HBcAb阳性组(小三阳组),常规体检中单独表面抗体阳性组。用ELISA法测血清中IL- 18、IL- 4、IL- 12、γ干扰素(IFN -γ)水平;生化分析仪测ALT、AST、TBIL、γGT浓度;荧光定量聚合酶链反应检测HBV DNA。结果　乙肝患者IL -18 水平明显高于对照组(P<0.01),以大三阳组水平最高,IL -18 与HBV DNA、HBeAg滴度、ALT、TBIL呈正相关,与rGT无相关性;IL- 18与IL- 4、IL- 12、IFN -γ均呈正相关;大三阳组与小三阳组中ALT与HBV DNA无相关性(P>0.01)。结论　HBV导致的肝脏损害是免疫应答引起的,在此过程中IL- 18 以网络形式发挥着双刃剑般平衡抗病毒与炎性损伤的作用。Objective To investigate the serum IL-18 level in patients with hepatitis B virus infection. Methods The patients were divided into 3 groups: HBsAg+, HBeAg+ and HBcAb+(A), HBsAg+,HBeAb+ and HBcAb+(B), healthy individuals with HBsAb+(as normal controls).Serum IL-18,IL-4,IL-12,IFN-γ level were determined by ELISA method in hepatitis patients and normal controls,ALT,AST,TBIL,gamm GT and HBV DNA were also determined.Results Concentration of serum IL-18 in patients with different types of viral hepatitis was significantly higher than that in healthy volunteers(P<0.01).The level of IL-18 in patients with A was the highest among those groups.Moreover,there was a close positive correlations among concentrations of serum ALT,TBIL,HBV DNA and other serum interleukins and IL-18,while HBV DNA and ALT were not.Conclusions The results show that IL-18 and other interleukins construct net effection and participate in live damage in patients with hepatitis B virus infection.

关 键 词：IL-18 HBV 细胞因子水平 乙型肝炎患者 ALT 阳性 TBIL DNA 表达水平 Γ-GT 

分 类 号：R512.62[医药卫生—内科学] R446[医药卫生—临床医学]