2种昆虫病毒混合悬液对菜粉蝶的增效作用  

Study on Synergetic Action of Two Kinds of Insect Virus to Pieria rapae (L.)

作　　者：王晓容[1] 匡石滋[1] 吴洁芳[1] 石尧清[1] 张慧[1] 连书亭 

机构地区：[1]广东省农业科学院果树研究所,广州510640 [2]河南省禹州市生物农药厂,禹州456570

出　　处：《华中农业大学学报》2005年第1期21-24,共4页Journal of Huazhong Agricultural University

基　　金：广东省星火计划项目(2003B21602);河南省高新技术产业化重点项目[豫高新(2001)号]资助

摘　　要：在温度为20～22℃的实验室条件下,用AfMNPV和PrGV的混合病毒悬液感染3龄期菜粉蝶幼虫,可有效降低2种病毒的用量,并显示出良好的增效作用,PrGV和AfMNPV毒力倍数分别为单剂的600倍和400倍以上.不同浓度混合病毒悬液的LT50分别比AfMNPV和PrGV单剂的LT50缩短1.10～2.06 d和0.40～1.93 d.用PrGV病毒悬液感染4龄期菜粉蝶幼虫,其LT50比3龄期增加3.43～6.06 d.Third-instar larvae of Pieria rapae (L.) were infected in combinations of the nucleopolyhedrovirus of Anagrapha falcifera Multiple nucleoplyhedrovirus(AfMNPV) and P. rapae granulosis virus(PrGV) under 20~22℃.The results showed that the mixture could reduce concentration of AfMNPV and PrGV which were only used efficaciously and enhance the effect obviously. The synergic ratio of PrGV and AfMNPV is over 600 and 400.The LT 50 of the virus mixture were shorter than AfMNPV and PrGV for 1.10～2.06 d and 0.40～1.93 d when the virus were used for control of P. rapae separately. When PrGV was studied for infection 4 instar larvae of P. rapae ,the LT 50 were added 3.43～ 6.06 d than LT 50 of 3 instar larvae.

关 键 词：菜粉蝶 颗粒体病毒 芹菜夜蛾核型多角体病毒 增效 

分 类 号：S476.13[农业科学—农业昆虫与害虫防治]