补阳还五汤对脑缺血再灌注后星形胶质细胞及热休克蛋白的影响  

Effect of Buyanghuanwutang on Astrocyte and Expression of Heat Shock Protein after Cerebral Ischemia Reperfusion

作　　者：谢建军 赖真[2] 耿小茵[2] 王耀邦[2] 王沙燕[2] 

机构地区：[1]广东省珠海市拱北医院 [2]暨南大学医学院附属二医院深圳市人民医院

出　　处：《中西医结合心脑血管病杂志》2005年第3期225-226,共2页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：广东省中医药局科研课题 (No .9961 7)

摘　　要：目的　探讨补阳还五汤对脑缺血再灌注后星形胶质细胞(AS)及热休克蛋白 (HSP70 )的影响。方法　采用沙土鼠双侧颈动脉夹闭脑缺血模型 ,于脑缺血 15min再灌注 2 4h和 48h后 ,运用免疫组化技术观察星形胶质纤维酸性蛋白 (GFAP)及热休克蛋白70的蛋白基因表达。结果　缺血再灌注 2 4h后 ,GFAP免疫阳性反应达高峰 ,补阳还五汤可使GFAP免疫反应减轻 ;缺血再灌注 48h后GFAP表达减弱 ,补阳还五汤可使其增强 ;缺血再灌注后 48h ,HSP70mRNA及蛋白表达明显增强 ,补阳还五汤可明显抑制其转录外 ,还可轻度降低HSP70的翻译。Objective To study the effect of Buyanghuanwutang(BYHWT) on astrocyte(AS) and the Expression of Heat Shock Protein(HSP70) after cerebral ischemia reperfusion.Methods Cerebral ischemia model in gerbils was produced by Ligating bilateral common carotid artery. The dynamic expression of GFAP and HSP70 were observed by immunochemistry after cerebral ischemia for 15 min followed by reperfusion for 24 hours and 48 hours.Results GFAP positive reactions reached a peak after cerebral ischemia for 15 min followed by reperfusion for 24 hours. BYHWT could decrease the expression. GFAP positive reactions decreased after cerebral ischemia for 15 min followed by reperfusion for 48 hours, BYHWT could increase the expression. HSP70 mRNA and the expression of HSP70 protein were increased obviously after cerebral ischemia for 15 min followed by reperfusion for 48 hours. BYHWT decreased the transcription of HSP70 mRNA remarkably and reduced slightly the expression of HSP70 protein.Conclusion The regulation of Radix on astrocytes after cerebral ischemia and reperfusion may be related with repairing process after cerebral ischemia.

关 键 词：补阳还五汤 脑缺血 星形胶质细胞 热休克蛋白 

分 类 号：R277.7[医药卫生—中医学]