非盐依赖层析的研究与应用  

The Development and Application of Salt-independent Chromatography

作　　者：韩凌[1] 胡洪波[1] 彭华松[1] 张雪洪[1] 

机构地区：[1]上海交通大学生命科学技术学院,上海200240

出　　处：《生物化学与生物物理进展》2005年第1期86-90,共5页Progress In Biochemistry and Biophysics

基　　金：上海交通大学青年教师基金资助项目~~

摘　　要：在非盐依赖层析操作中,吸附介质的成分、结构、密度等性质得到改进,所以料液离子强度的变化不会明显影响吸附. 与常用的离子交换层析、疏水层析、亲硫层析等方法相比,该类技术能够降低对料液预处理的要求,提高蛋白质的稳定性,同时简化层析操作、降低纯化成本,具有大规模分离纯化蛋白质的潜力. 近年来开发的多种非盐依赖层析介质与方法,都已在蛋白质纯化中得到应用.In the salt-independent chromatography, the component, structure, density and some other characteristics of the adsorption media are improved, so the variations of feedstock ion strength have no significant effect on adsorption. The great advantage of the salt-independent is absent in the traditional chromatography methods such as the ion-exchange chromatography, the thiophilic chromatography and the hydrophobic chromatography. This kind of technologies can lower the demands on preparation of the crude feedstock, therefore the steps of desalting, diluting or adding the extra salts could be bypassed. It simplifies the process and makes it more economical, so it is compatible with large scale requirements of the separation of the proteins, especially the enzyme and the antibody. The recent development of the new ligands and methods on salt-independent chromatography are summarized.

关 键 词：层析 非盐依赖 混合模式 疏水电荷诱导 配基 

分 类 号：O658.1[理学—分析化学]