一个与大豆黄色种皮相关的RAPD标记  

A RAPD MARKER RELATED WITH YELLOW SEED COAT OF SOYBEAN

作　　者：王惠[1] 段玉玺[1] 陈立杰[1] 邱丽娟[2] 常汝镇[2] 

机构地区：[1]沈阳农业大学植物线虫学研究室,沈阳110161 [2]中国农科院作物所农业部作物种质资源与生物技术重点开放实验室,北京100081

出　　处：《大豆科学》2005年第1期17-20,共4页Soybean Science

基　　金：国家自然科学基金课题(39770494)资助

摘　　要：　利用BSA法对11个黑色种皮的大豆品种和12个黄色种皮的大豆材料的基因组DNA进行了RAPD分析,供试的100个随机引物有 66 个产生了 RAPD扩增产物,其中产生 RAPD多态性的随机引物有15个。在所产生的扩增产物中,获得了一个与大豆种皮色相关的特异 DNA 片段S79500。同时,采用该标记对辽豆10号×PI437654杂交后代种皮颜色有分化的群体和黄、青、黑、褐色种皮的大豆进行检测,经验证这个 RAPD标记具有较高的重复性和稳定性,可用于辅助选育优良的黄色种皮的大豆新品种。BSA method was employed to analyze genome DNA of 11 black coat seed soybean and 12 yellow coat seed soybean by RAPD method. The bands were produced for 66 primers among 100 primers. There were polymorphism bands for 15 primers. In all products amplified, a special DNA fragment , S79500 was closely related with the yellow coat of soybeans. The primer S79500 became a RAPD marker for the yellow coat of soybean. In another hand, the S79500 also was used to test the filial generation with yellow, green, black and brown coat from Liaodou 10×PI437654. The results showed that the S79500 was very stable and repeatable. The RAPD marker S79500 was useful to assistant the selection for soybean hybrid breeding.

关 键 词：种皮 大豆 RAPD标记 随机引物 RAPD多态性 杂交后代 RAPD分析 辅助 分化 皮色 

分 类 号：S565.1[农业科学—作物学] S531