八氢番茄红素合成酶基因(PSY)对大豆的遗传转化  

GENETIC TRANSFORMATION OF PHYTOENE SYNTHASE GENE(PSY)INTO SOYBEAN

作　　者：龚学臣[1] 季静[1] 抗艳红[2] 王罡[1] 吴颖[3] 王萍[3] 

机构地区：[1]天津大学农业与生物工程学院,天津300072 [2]河北北方学院农业科学系,张家口075131 [3]淮海工学院海洋学院,连运港222005

出　　处：《大豆科学》2005年第1期30-33,共4页Soybean Science

基　　金："国家植物转基因技术研究开发与中试基地建设"专题课题(项目编号199-B001)

摘　　要：　以三个大豆品种的无菌苗子叶节为受体,以 PSY为目的基因,应用根癌农杆菌介导法对大豆进行了遗传转化,经子叶节丛生芽诱导、茎伸长培养、生根培养和潮霉素选择培养,获得了完整的抗性再生苗,经PCR和PCR-Southern分析鉴定,初步证明外源基因 PSY已整合到大豆的基因组中,并对影响遗传转化的因子进行了研究。Taking cotyledonary nodes of three varieties of soybean as acceptor, PSY gene were transformed into soybean via agrobacterium-mediated. Hygromycin resistant regeneration plants of soybean were obtained by cotyledonary nodes inducting, striking root cultivation and stem elongation cultivation and hygromycin screening in selective condition. PCR and PCR-Southern analysis from the transferred gene plants proved that PSY has been transferred into genome of soybean. The factors affecting genetic transformation were also investigated.

关 键 词：遗传转化 大豆 子叶节 潮霉素 丛生芽诱导 根癌农杆菌介导法 再生苗 酶基因 受体 番茄红素 

分 类 号：S565.1[农业科学—作物学] S572