前列腺移行带和外周带差异基因表达研究  

Expression of different genes in transitional zone and peripheral zone of human normal prostate

作　　者：孙宏斌[1] 夏术阶[1] 唐孝达[1] 

机构地区：[1]上海交通大学附属第一人民医院泌尿外科,200080

出　　处：《中华医学杂志》2005年第9期610-613,共4页National Medical Journal of China

摘　　要：目的研究正常前列腺移行带和外周带在基因表达上存在的差异。方法二步法提取移行带和外周带组织总RNA,应用含有6700条人cDNA微距阵基因芯片,分别检测17例正常前列腺移行带和外周带的基因表达谱并重复3次,用cy5dUTP标记移行带组织mRNA,cy3dUTP标记周围带组织mRNA。Ratio均数(RA)<0.5或>2入选为表达差异的基因,RA<0.2或>4判断为表达有极显著差异的基因。采用RT PCR法验证差异表达的基因。结果移行带和外周带有表达差异的基因共640条(移行带294条,外周带346条),在GeneBank中功能明确者371条。表达有极显著差异的基因14条,其中移行带高表达的有表皮生长因子(EGF),外周带高表达的有成骨细胞刺激因子1(OSF1)。RT PCR结果证明在17例正常前列腺移行带和20例良性前列腺增生尿道周围组织中,EGF与内参基因β肌动蛋白的相对表达量明显高于外周带。结论正常前列腺移行带和外周带存在着基因表达差异,这可能是两带性结构差异生物学行为的分子基础;EGF对移行带的生长有重要促进作用。Objective To detect the expression of different genes in the transitional zone and peripheral zone of human normal prostate. Methods Seventeen specimens of normal prostate gland were obtained from dead kidney donors and patients undergoing total cystectomy. The transitional zone and peripheral zone were isolated. Twenty specimens of benign prostate hypertrophy (BPH) were obtained from patients undergoing prostatic enucleation. The periurethral significantly hypertrophic nodules were isolated. BioDoor Chip-40S containing 6700 clones of targeting gene cDNA was used to detect the gene expression. The peripheral zone RNA samples were labeled with cy3, and the transitional zone RNA samples labeled with Cy5. The genes with the ratio average (RA) <0.5 or >2 were identified as differentially expressed genes, and those with a RA<0.2 or >4 were identified as the most significantly differentially expressed genes. RT-PCR was used to investigate semi-quantitatively the expression of epidermal growth factor (EGF) mRNA in the 17 specimens of transitional zone and peripheral zone from the 17 normal prostate glands and the 20 specimens of periurethral zone from BPH. Results A total of 640 genes differentially expressed in transitional zone and peripheral zone were screened out (comprising 269 unknown genes and 371 known genes), of which 294 genes were expressed in the transitional zone and 346 in the peripheral zone. Fourteen genes were identified as markedly differentially expressed, among which the EGF gene was highly expressed in the central zone, and the OSF-1 (osteoblast stimulating factor-1) gene was highly expressed in the peripheral zone. RT-PCR proved that in comparison with the expression of the internal marker β-actin gene, the expression levels of EGF in the normal transitional zone and in the periurethral zone from BPH were markedly higher than that in the peripheral zone. Conclusion There are differently expressed genes between the peripheral zone and central zone of normal human prostate, which may be the backgrou

关 键 词：外周 移行 前列腺 正常 基因 EGF RA RNA 表达差异 微距 

分 类 号：R697.3[医药卫生—泌尿科学]