去卵巢大鼠胫骨形态计量学与一氧化氮的关系  

Relationship between nitric oxide and tibia histomorphometry in ovariectomised rats

作　　者：石少辉[1] 章明放[1] 张文治[2] 邱爽[3] 李晋平[1] 

机构地区：[1]天津医科大学病理教研室,300070 [2]天津市环湖医院 [3]天津医科大学免疫教研室

出　　处：《中国骨质疏松杂志》2005年第1期42-44,共3页Chinese Journal of Osteoporosis

摘　　要：目的　初步探讨去势后不同时期大鼠骨髓基质细胞上清液一氧化氮 (NO)与胫骨形态计量学变化的关系。方法　 4月龄雌性Wistar大鼠 36只 ,随机分为 6组 :假切 1月组 (Ⅰ组 ) ,假切 4月组 (Ⅱ组 ) ,去势 1月组 (Ⅲ组 ) ,去势 4月组 (Ⅳ组 ) ,去势 7月组 (Ⅴ组 ) ,去势 12月组 (Ⅵ组 )。各组大鼠处死后取左侧胫骨、股骨体外培养骨髓基质细胞 ,测定各组大鼠上清液中NO浓度的改变 ;取右侧胫骨近端制备不脱钙的骨切片 ,观察各组大鼠胫骨形态计量学的变化。结果　与假切对照组比较去势组大鼠上清液中NO浓度降低 ,平均骨小梁体积减小 ,成骨表面和类骨质表面等成骨指数降低 ;破骨细胞、吸收表面等骨吸收指数升高 ;去势 4、7、12月组NO浓度改变无统计学意义。结论　在骨质疏松患者骨的代谢过程中破骨细胞的数目增多 ,活性增强与NO浓度改变有关。Objective To investigate the relationship between the changes in nitric oxide (NO) and the tibia in ovariectomized rats. Methods Sixty-six female Wistar rats were divided into six groups randomly. The first group was sham-ovariectomized for one month, and the second group for four months. The third group was ovariectomized for one month, and the fourth for four months, the fifth for seven months, and the sixth for one year. All rats were killed at the end of experiment. Their right proximal tibia were processed into undecalcified sections, and the bone narrow stromal cells of left tibia and femora were cultured in vitro. Then the concentration of NO in supernatant of culture and the changes in histomorphometry were examined. Results The NO concentration and trabecular bone volume in ovariectomized groups were significantly lower, and the osteoblast surface and the osteotoid surface were smaller than those in sham-operated groups, while the number of osteoclasts and erosion surface were significantly greater. Conclusions NO plays an important role in bone resorption, and may be related to that the increase in number and the enhancement in activity of osteoclasts during osteoporosis.

关 键 词：胫骨 骨形态计量学 NO浓度 去卵巢大鼠 改变 破骨细胞 骨髓基质细胞 一氧化氮 上清液 去势 

分 类 号：R681[医药卫生—骨科学] R683[医药卫生—外科学]