低G锻炼对复合应激致大鼠学习记忆障碍的保护作用  

Protective effect of lower G training on compound stress induced learning and memory impairment in rats

作　　者：刘挺松[1] 孙喜庆[1] 曹新生[1] 吴兴裕 

机构地区：[1]第四军医大学航空航天医学系航空航天生物动力学教研室,西安710032

出　　处：《中华航空航天医学杂志》2004年第3期146-150,共5页Chinese Journal of Aerospace Medicine

基　　金：军队十五指令性课题 (0 1L0 72 )

摘　　要：目的　探讨低G锻炼对加速度和噪声复合应激致大鼠学习记忆能力障碍的保护作用。　方法　 2 4只雄性SD大鼠随机分为对照组、复合应激组和锻炼组 ,每组 8只。复合应激组大鼠采取+10Gz/3min复合 90dB(A)、10 0 0Hz/30min噪声暴露 ,复合方式为从噪声作用的第 2 7min开始叠加加速度。锻炼组大鼠在复合应激暴露之前连续 3d(1次 /d)进行 +4Gz/3min暴露 ,第 4天接受和复合应激组同样的暴露。采用Y 型迷宫实验和避暗实验观察应激和锻炼后不同时间大鼠学习记忆能力的变化。　结果　复合应激组大鼠反应时在暴露后各时间点较对照组均显著延长 (P <0 0 1) ,正确率均显著下降 (P <0 0 1) ;锻炼组大鼠反应时仅在暴露后即刻较对照组显著延长 (P <0 0 5 ) ,而较复合应激组在各时间点均显著缩短 (P <0 0 1) ,锻炼组正确率较复合应激组在暴露后各时间点显著升高 (P <0 0 1)。复合应激组大鼠总时间和错误数较对照组显著增加 (P <0 0 1) ,潜伏期显著缩短(P <0 0 1)。锻炼组大鼠总时间和错误数较复合应激组显著减少 (P <0 0 1) ,潜伏期显著延长 (P <0 0 1)。　结论　连续 3d的 +4Gz/3min(1次 /d)锻炼对复合应激 (+10Gz/3min、90dB(A) /30min)致大鼠学习记忆功能障碍具有一定的保护作用。Objective To explore the protective effect of lower +G z training on compound stress induced learning and memory impairment in rats. Methods Twenty four male SD rats were randomly divided into control group, compound stress group and training group. Rats of compound stress group were exposed to noise [1000 Hz, 90 dB(A)] for 30 min and +10 G z for 3 min. Rats of training group underwent +4 G z training (+4 G z for 3 min per day) for 3 successive days and then exposedto compound stress on the fourth day. Learning and memory of rats were measured by means of Y-maze test and step-through test immediately, 2 d, 4 d, 6 d after compound stress exposure. Results Compared with control group, reaction time increased significantly (P<0.01) and percentageof right reaction decreased significantly (P<0.01) in rats of compound stress group on all occasions measured after stress. Reaction time of rats in training group was significantly (P<0.05) longer immediately after stress as compared with control group, but significantly (P<0.01) shorter as compared with compound stress group. Compared with compound stress group, percentage of right reaction in training group was significantly (P<0.01) higher on all occasions measured after stress. Compared with control group, total time and numbers of errors in training group increased significantly(P<0.01) and latent time decreased significantly (P<0.01). Compared with compound stress group, total time and numbers of errors in training group decreased significantly (P<0.01) and latent time increased significantly (P<0.01). Conclusions Lower +G z training may provide protectiveeffect on compound stress induced learning and memory impairment in rats.

关 键 词：噪声 大鼠 低G锻炼 战斗机 飞行员 正加速度 复合应激 学习记忆障碍 

分 类 号：R82[医药卫生—临床医学]