Isozyme Analysis of Spotted Halibut Verasper variegatus Temminck et Schlegel  

作　　者：LIUManhong GAOTianxiang ZHANGXiumei CHENSiqing 

机构地区：[1]TheKeyLaboratoryofMariculture,MinistryofEducation,OceanUniversityofChina,Qingdao266003,P.R.China [2]TheKeyLaboratoryofMariculture,MinistryofEducation,OceanUniversityofChina,Qingdao266003,P.R.China／／YellowSeaF~sheriesResearchInstitute,ChineseAcademyofFisherySciences,Qingdao266071,P.R.China

出　　处：《Journal of Ocean University of China》2005年第1期43-48,共6页中国海洋大学学报（英文版）

基　　金：the 863 High-Technology Program(2001AA621020).

摘　　要：To investigate the tissue-specificities of isozymes and the genetic structureof wild spotted halibut ( Verasper variegatus) population, horizontal starch gel electrophoresiswas performed on 45 individuals collected in part of the Yellow Sea. The performances of 17 isozymesin 8 kinds of tissues or organs were screened preliminarily in a TC-7.0 buffer system. The resultsshowed that the screened isozymes displayed remarkable tissue-specificities. Finally, 14 enzymes(AAT, ADH, EST, OPI, G3PDH, IDHP, LAP, LDH, MDH, MPI, PGDH, PGM, SDH and SOD) and 4 kinds of tissues(eye, skeleton muscle, liver and heart) were selected for genetic analysis. Fourteen isozymes areencoded by 20 loci, and 9 of them are polymorphic. The polymorphic loci are AAT-1~*, GPI-2~*,G3PDH~*, IDHP-1~*, LDH~*, MPI~*, PGM-1~*. PGM-2~* and SDH~*, and the proportion of polymorphic lociis 0.4500 (P_(0.99)) ? The mean values of observed and expected heterozygosities are 0.0278and 0.0265, respectively and the average effective number of alleles is 1.0675.To investigate the tissue-specificities of isozymes and the genetic structure of wild spotted halibut ( Verasper varie- gatus) population, horizontal starch gel electrophoresis was performed on 45 individuals collected in part of the Yellow Sea. The performances of 17 isozymes in 8 kinds of tissues or organs were screened preliminarily in a TC-7.0 buffer system. The results showed that the screened isozymes displayed remarkable tissue-specificities. Finally, 14 enzymes (AAT, ADH, EST, GPI, G3PDH, IDHP, LAP, LDH, MDH, MPI, PGDH, PGM, SDH and SOD) and 4 kinds of tissues (eye, skeleton mus- cle, liver and heart) were selected for genetic analysis. Fourteen isozymes are encoded by 20 loci, and 9 of them are poly- morphic. The polymorphic loci are AAT-1*, GPI-2*, G3PDH*, IDHP-1*, LDH*, MPI*, PGM-1*, PGM-2* and SDH*, and the proportion of polymorphic loci is 0.4500 (P0.99). The mean values of observed and expected heterozygosities are 0.027 8 and 0.026 5, respectively and the average effective number of alleles is 1.067 5.

关 键 词：verasper variegatus ISOZYME TISSUE-SPECIFICITY genetic structure 

分 类 号：Q959.4[生物学—动物学] Q5