间日疟原虫不同MSP-1等位基因型形态学观察  

Morphological observation on the genotypes of the merozoite surface protein-1 alleles of Plasmodium vivax

作　　者：张山鹰[1] 许龙善[1] 张莹珍[1] 谢汉国[1] 高琪[2] 陆惠民[3] 

机构地区：[1]福建省疾病预防控制中心,福州35000 [2]江苏省寄生虫病防治研究所 [3]苏州大学医学院寄生虫学教研室

出　　处：《中国人兽共患病杂志》2005年第3期234-236,205,共4页Chinese Journal of Zoonoses

基　　金：福建省医学创新课题 (2 0 0 3 -cx -0 4)

摘　　要：目的　比较两种不同等位基因型间日疟原虫生物学形态特征。方法　现场采集间日疟病人血样并进行基因分型 ,镜下观察形态并测量大小。结果与结论　 72份现场分离株基因分型为Sal- 1型 4 0份 ,Belem型 2 5份 ,混合感染 7份 ,均查到典型的间日疟原虫 ,6例多重感染病例均发现在海南分离株 ,多重感染与基因型混合感染无关联。测量正常红细胞、寄生红细胞、环状体以及核大小差别有显著意义 ,Belem型比Sal- 1型大 。To compare the morphological characteristics of two merozoite surface protein-1 (MSP-1) alleles of Plasmodium (vivax), blood samples of malaria patients confirmed by morphological examinations were collected and subjected to genotyping with nested PCR. The size of plasmodia was measured and calculated by micro-measure device. The results in genotyping showed that of the 72 field isolates, 40 isolates belonged to Sal-1 type. Twenty-five isolates to Belem type and 7 isolates were mixed types. The typical morphology of P.vivax was demonstrated. Six cases of multiple infection( more than two parasites in one red cell) were all found in the isolates from Hainan province with no significant correlation between mixed genotypes and multiple infection of red cells. There were significant differences between Sal-1 and Belem types in the sizes of normal and infected red cells, ring forms and their nuclei. As to the molecular mechanisms in the different genotypes, further investigations are needed.

关 键 词：间日疟原虫 裂殖子表面蛋白1 基因型 形态学 

分 类 号：R382.3[医药卫生—医学寄生虫学]