节节麦与黑麦间杂种F1胚再生植株及其双二倍体细胞遗传学分析  

Research on Cytogenetics of the Hybrid Embryo Regeneted F1 and Amphidiploid Between Triticum Tauschii and Secale Cereale

作　　者：石永春[1] 裴冬丽[1] 李锁平[1] 

机构地区：[1]河南大学生命科学院,河南开封475001

出　　处：《河南大学学报（自然科学版）》2005年第1期58-61,共4页Journal of Henan University:Natural Science

基　　金：河南省杰出青年科学基金项目(02120000900)

摘　　要：将节节麦与黑麦杂交,利用F1胚愈伤组织培养再生杂种植株,对胚再生杂种F1进行秋水仙素加倍获得了节节麦和黑麦的双二倍体(DDRR). 杂种F1(DR)的减数分裂中期Ⅰ (PMCMI)染色体构型平均为 2n=10. 16Ⅰ +1. 86Ⅱ+0. 12Ⅲ+0. 00625Ⅳ. 该双二倍体(DDRR)的减数分裂中期Ⅰ(PMCMI)染色体构型平均为 2n=8. 676Ⅰ +9. 318Ⅱ+0. 019Ⅲ+0. 004Ⅳ,C1的结实率平均为 2. 118%. 该双二倍体可作为一个新的物种以及作为改良小麦的种质材料.Amphidiploid(2n=28,DDRR) of Triticum tauschii and Secale cereale was synthesized through culture of immature hybrid embryos and colchicines treatment. Through the researching to the PMC MI chromosome pairing configurations of the embryo regeneted F1 and the amphidiploid were 10.16Ⅰ+1.86Ⅱ+0.12Ⅲ+0.00625Ⅳ and 8.676Ⅰ+9.318Ⅱ+0.019Ⅲ+0.004Ⅳ, respectively.The mean seed sets of the amphidiploid C1 was 2.118%, which could be used as a new speices and materials to improve wheat.

关 键 词：节节麦 黑麦 双二倍体 

分 类 号：Q32[生物学—遗传学]