加热对人前列腺增生上皮细胞的作用  

Effects of Heating on the Epithelia of Benign Prostate Hyperplasia in vitro

作　　者：孙明[1] 杨宇如[1] 李虹[1] 卢一平[1] 魏强[1] 沈宏[1] 石明[1] 

机构地区：[1]四川大学华西医院泌尿外科,四川成都610041

出　　处：《中华男科学杂志》2005年第3期213-216,共4页National Journal of Andrology

摘　　要：目的: 探讨不同温度作用下及作用后不同时间人前列腺增生上皮细胞的生物学特性变化。　方法: 原代培养前列腺增生上皮细胞,经 45℃、60℃分别加热 15min后继续培养 1h和 12h,行细胞增殖实验、细胞形态学观察、流式细胞仪分析、热休克蛋白 70(HSP70)和前列腺特异性抗原 (PSA)的免疫细胞化学检测。　结果: 45℃组作用后以细胞凋亡为主, 60℃组以细胞坏死为主。两组作用后均有显著的生长抑制作用, 60℃组较 45℃组显著(P<0. 01)。加热主要导致G0 /G1期为主的细胞周期阻滞,主要在加热后 12h组出现 (P<0. 01)。加热后HSP70和PSA的表达有显著的上调(P<0. 01)。　结论: 加热可以致前列腺增生上皮细胞凋亡、坏死和生长抑制,其作用机制与加热阻滞细胞周期、上调HSP70和PSA的表达相关。Objective: To invetigate the biological changes of the epithelia of benign prostate hyperplasia under heating at different temperatures and at different points of time after heating. Methods: Cell morphology, MTT, flow cytometer, and the immunocytochemical method for detecting heat shock protein 70 and prostate specific antigen were used to observe the effect of heating on the primarily cultured epithelia of benign prostate hyperplasia at 45 ℃ and 60 ℃ and at 1 hour and 12 hours after heating. Results: Heating at 45 ℃ for 15 min resulted in apoptosis, and at 60 ℃, necrosis in most of the cells. The inhibiting effect of heating on the growth of cells was observed, more significant in the 60 ℃ group than in the 45 ℃ group. The cell phase arrest induced by heat, mainly G 0/G 1 arrest, was more significant in the 12 h group than in the 1 h group. Heating up-regulated the expressions of heat shock protein 70 and prostate specific antigen in cells. Conclusion: Heating can induce apoptosis, necrosis and growth suppression of the epithelia of benign prostate hyperplasia. Its process and mechanism are correlated with the cell phase arrest and the up-regulation of the expressions of heat shock protein 70 and prostate specific antigen.

关 键 词：前列腺增生 加热 上皮细胞 人 

分 类 号：R697.3[医药卫生—泌尿科学]