rhIL-11对正常IEC-6细胞的增殖及细胞周期的影响  

Effect of recombinant human interleukin-11 on proliferation and cell cycle of normal IEC-6 cells

作　　者：余祖胤[1] 熊国林[1] 刘耀文[1] 黄海潇[1] 谢玲[1] 董波[1] 王欣茹 罗庆良[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850 [2]二炮总医院检验科

出　　处：《解放军医学杂志》2005年第3期191-193,共3页Medical Journal of Chinese People's Liberation Army

基　　金：全军医学科研"十五"计划指令性课题资助项目(编号01L019)

摘　　要：目的　通过研究重组人白介素11(rhIL-11)对正常IEC-6细胞增殖、细胞周期及其IL -1受体表达的影响,探讨IL -1对 小肠上皮细胞的调控作用以及相关机制。方法　利用MTT法检测rhIL -1对正常IEC-6细胞增殖的影响,通过流式细胞仪检测细 胞周期的变化,并采用Westernblot方法检测IEC-6细胞表面IL-11受体α链的表达情况。结果　rhIL-11可引起正常IEC-6细胞发 生增殖抑制和明显的G0/G1期阻滞,并诱导细胞表面IL 11受体α链表达增加。结论　rhIL-11可能通过与IEC-6细胞表面特异性受 体结合,从而启动胞浆内信号转导通路,引起细胞效应。Objective In order to investigate the effects of interleukin-11 (IL-11) on the small intestinal epithelial cells, we observed the effect of recombinant human interleukin-11 on the proliferation, cell cycle and the expression of IL-11 receptor of IEC-6 cells in vitro. Methods The cell cycle, proliferation and the expression of IL-11 receptor of IEC-6 cells were determined by means of MTT, flow cytometry and Western blot. Results The results demonstrated that rhIL-11 could induce proliferative inhibition and cell cycle retardation in normal IEC-6 cells. Moreover, rhIL-11 also evoked the expression of alpha-chain protein of IL-11 receptor in IEC-6 cells. Conclusion These results implicated that rhIL-11 might affect the IEC-6 cells by combining with the receptor on the cell membrane to regulate the signal transduction pathway in the cytoplasm.

关 键 词：白细胞介素11 细胞增殖 细胞周期 

分 类 号：Q253[生物学—细胞生物学]