rhIL-11对中子照射小鼠肠损伤的治疗作用  

Effect of recombinant human interleukin-11 on neutron irradiation injury of small intestine in mice

作　　者：王欣茹 余祖胤[2] 黄海潇[2] 熊国林[2] 王宝勤[2] 谢玲[2] 刘耀文[2] 高亚兵[2] 彭瑞云[2] 罗庆良[2] 

机构地区：[1]二炮总医院检验科,北京100088 [2]军事医学科学院放射医学研究所

出　　处：《解放军医学杂志》2005年第3期201-203,共3页Medical Journal of Chinese People's Liberation Army

基　　金：军队医学科研"十五"计划指令性课题资助项目(编号 01L019)

摘　　要：目的　研究重组人白介素11(rhIL-11)对中子照射小鼠小肠上皮损伤及细胞周期的影响。方法　采用裂变中子照射小 鼠模型,观察rhIL 11对照射后小鼠小肠上皮形态、隐窝细胞坏死和细胞增生的影响,用流式细胞仪检测小鼠小肠上皮细胞周期的变 化。结果　rhIL-11治疗和预防给药可通过促进小肠隐窝细胞增殖使肠损伤得到迅速修复。照射后小鼠小肠上皮细胞发生明显的 G2期阻滞,rhIL-11治疗可明显提高S期细胞比例。结论　rhIL -1对中子照射小鼠小肠损伤具有防护作用,并能够影响细胞周期的 变化。Objective To evaluate the effect of rhIL-11 on radiation injury to the intestinal epithelium and cells cycle of intestinal epithelial cell in mice irradiated with 3.5 Gy neutron. Methods The morphology of the small intestinal epithelium, crypt cells necrosis, and cell proliferation were observed of the epithelial cells of the irradiated mice. Cell cycle of the epithelial cells of the small intestine of the mice was examined by flow cytometry. Results rhIL-11 pretreatment before and treatment after irradiation could accelerate the repair of small intestinal mucosa in irradiated mice. G 2/M block which occurred in the irradiated small intestinal epithelial cells and the rhIL-11 treatment might significantly increased the proportion of cells at S phase. Conclusion rhIL-11 could significantly exert a preventive effect on the small intestine against radiation injury in neutron irradiated mice, with an impact on cell cycle of the intestinal epithelial cells.

关 键 词：白细胞介素11 中子 辐射损伤 实验性 小肠 细胞周期 

分 类 号：Q253[生物学—细胞生物学]