rhIL-11+rhG-CSF对中子急性放射病狗的治疗作用  

Therapeutic effect of combination of rhIL-11 and rhG-CSF on acute radiation sickness produced by neutron irradiation in dogs

作　　者：王欣茹 黄海潇[2] 熊国林[2] 谢玲[2] 余祖胤[2] 王宝勤[2] 罗庆良[2] 

机构地区：[1]二炮总医院检验科,北京100088 [2]军事医学科学院放射医学研究所

出　　处：《解放军医学杂志》2005年第3期204-207,共4页Medical Journal of Chinese People's Liberation Army

基　　金：全军医学科研"十五"计划指令课题资助项目(编号01L019)

摘　　要：目的　探讨rhIL-11与rhG-CSF联用对中子急性放射病(ARS)的治疗作用。方法　18只雄性成年比格狗,随机分为照 射对照组、对症治疗组和两因子+对症联合治疗组。所有动物均接受90%中子照射,吸收剂量为2.0Gy。联合治疗组动物于照射后 即刻开始皮下注射rhG-CSF10μg/(kg·d)和rhIL-1150μg/(kg·d),给药时间分别为14天和21天,观察照射后动物外周血象及骨髓 有核细胞形成CFU-GM能力的变化。结果　对症治疗组和联合治疗组动物全部存活,而照射对照组动物30天存活率为57.1%。照 射后联合治疗组各检测点外周血白细胞数及恢复期血小板和红细胞数均明显高于照射对照组及对症治疗组。照射后1天,联合治疗 组骨髓CFU-GM数量是两个对照组的6倍(P<0.01),33天分别是照射对照组、对症治疗组的1.75和1.46倍(P<0.01)。结论　 rhIL-11与rhG-CSF联合给药可以升高2.0Gy裂变中子照射狗的外周血白细胞、血小板数的最低值,缩短白细胞最低值持续的时间, 加速骨髓造血功能的恢复,对2.0Gy中子照射狗有明显的治疗作用。Objective To evaluate the therapeutic effects of combined recombinant human IL-11(rhIL-11) and recombinant human G-CSF (rhG-CSF) on the neutron-irradiated dogs. Methods 18 male beagle dogs were divided into radiation control group (C, n=7), symptomatic treatment group (S, n=4), and combination therapy group (T, n=7). All dogs were exposed to total body 2Gy 90% neutron radiation. From the first day after radiation, the animals of group T received rhG-CSF 10μg/(kg·d) and rhIL-11 50μg/(kg·d) subeutaneously for 14d and 21d respectively. The cell counts of peripheral blood and CFU-GM of bone marrow were carried out. Results All animals of group S and T survived, however, the survival rate of group C was only 57%. The cellcounts of T group peripheral blood cells (white blood cell at any time point , the platelet and red blood cell of recovery phase) were higher than that of C or S group. The count of T group bone marrow CFU-GM was 6 fold higher than that of group C or S on day 1, and still 1.75, 1.46 fold higher than that group C or S, respectively, on day 33. Conclusion the combination therapy of rhIL-11 and rhG-CSF significantly raised the white cell and platelet counts in ARS dogs induced by neutron irradiation by accelerating the recovery of bone marrow hematopoietic function.

关 键 词：白细胞介素11 粒细胞集落刺激因子 重组 辐射损伤 实验性 中子 

分 类 号：R827.35[医药卫生—临床医学]