实验性肺纤维化细胞凋亡及Fas/FasL基因变化  

Cell apoptosis and the change of Fas/FasL gene in experimental pulmonary fibrosis

作　　者：李今朝[1] 李艳杰[1] 李振华[1] 康健[1] 侯显明[1] 于润江[1] 

机构地区：[1]中国医科大学附属第一医院呼吸疾病研究所,沈阳110001

出　　处：《中华结核和呼吸杂志》2005年第3期184-187,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的　检测肺纤维化时细胞凋亡及Fas/FasL基因的变化,探讨细胞凋亡和基因变化在肺间质纤维化中的意义。方法　将 40只大鼠分为肺纤维化组及正常对照组,每组 20只,应用末端原位杂交(TUNEL)、原位杂交、免疫组化技术检测博莱霉素致肺间质纤维化大鼠肺组织细胞凋亡、Fas/FasLmRNA水平及蛋白表达的变化。结果　肺纤维化组大鼠肺组织中细胞凋亡指数为 55.3±12.2,对照组为 4 7±1 0,两组比较差异有统计学意义(t=13.06,P<0 .01),Fas及FasLmRNA肺纤维化组为(175.8±21.6, 5.2±1.6),正常对照组为 (26.6±1.9, 0.5±0.4),两组比较差异有统计学意义 (t分别为 21.7, 8.79;P均<0.01),肺纤维化组Fas、FasL蛋白表达分别为 956±96, 285±76,正常对照组分别为 491±96, 100±18,两组比较差异有统计学意义 (t=5.03, 12.81;P<0.01)。结论　肺间质纤维化时肺组织细胞凋亡上调,Fas/FasL基因上调蛋白表达增强,在肺纤维化发生、发展中起到重要的作用。Objective To investigate the changes and significance of cell apoptosis and Fas/FasL gene in pulmonary fibrosis. Methods Forty mice were divided into two groups randomly, each group contained twenty mice. TUNEL,immunochistochemistry and in situ hybridization were used to detect the change of cell apoptosis,Fas/FasL mRNA and protein in mice with pulmonary fibrosis caused by bleomycin. Results The apoptosis index of lung cells in the pulmonary fibrosis group(55.3±12.2) was higher than that of control group((4.7±1.0, t=13.06,)P<0.01).The expression of Fas/FasL mRNA(175.8±21.6,5.2±1.6)and protein (956±96,285±76) in the pulmonary fibrosis group was higher than that of control group((mRNA: 26.6±1.9, 0.5±0.4, t=21.7,)8.79,all P<0.01; protein: 491±96, 100±18, t=5.03, 12.81, P<0.01).Conclusion The apoptosis index of lung cells, Fas/FasL genes and protein were up-regulated in lung tissue of pulmonary fibrosis, which may play an important role in the development of disease.

关 键 词：医学实验 肺纤维化 细胞凋亡 FAS/FASL基因 巨噬细胞 中性粒细胞 

分 类 号：R563.9[医药卫生—呼吸系统]