砷中毒患者皮肤组织中DNA修复基因的表达变化  

Study on the expression of DNA repair gene MGMT、XRCC1、hMSH2 mRNA in skin of patients with endemic arsenism caused by coal-burning

作　　者：张爱华[1] 李健[1] 潘雪莉[1] 蒋宪瑶[1] 岑笃才[2] 黄晓欣[2] 

机构地区：[1]贵阳医学院公共卫生学院,贵阳550004 [2]解放军第44医院

出　　处：《中国地方病学杂志》2005年第2期121-123,共3页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金资助项目(30460123)

摘　　要：目的检测DNA修复基因MGMT、XRCC1、hMSH2mRNA在燃煤型砷中毒患者皮肤组织中的表达变化,探讨其表达与砷性皮肤癌发生发展及临床病理特征之间的关系。方法应用原位杂交技术检测了61例砷中毒患者皮肤组织中MGMT、XRCC1、hMSH2mRNA的表达变化。结果随着砷中毒患者皮肤病变的发展,MGMT、XRCC1、hMSH2mRNA的表达逐渐降低,癌变组MGMT和XRCC1mRNA阳性表达率与一般病变组比较差异有统计学意义(P<0.05、P<0.01)。结论MGMT、XRCC1、hMSH2作为DNA损伤修复基因,在砷性皮肤病变过程中起重要作用;砷可能通过抑制砷中毒患者皮肤组织中MGMT、XRCC1等DNA修复基因的表达,影响基因组DNA稳定性和DNA修复功能而导致对皮肤的致癌作用。Objective To study the expressions of DNA repair genes MGMT,XRCC1,hMSH2 mRNA in skin of patients with endemic arsenism caused by coal-burning and to evaluate their roles in shin cancer progression. Methods 61 cases of patients with arsenism caused by coal-burning pollution were examined by using in situ hybridization for the expressions of MGMT,XRCC1,hMSH2 mRNA. Results The positive rates of MGMT,XRCC1,hMSH2 mRNA in skin were gradually decreased as the skin pathological changes grew worse. There were significant changes of expressions of MGMT and XRCC1 mRNA in skin carcinoma group compared with the usual pathological change group (P < 0.05, P < 0.01). Conclusions As DNA repair genes, MGMT,XRCC1 and hMSH2 may play importance roles in skin arsenism. Arsenic causes carcinogenicity on human skin through inhibiting the expressions of MGMT,XRCC1 and influencing the genetic stability and the DNA repair function.

关 键 词：XRCC1 患者 MGMT 砷中毒 皮肤组织 表达 DNA修复基因 RNA 基因组DNA DNA损伤修复 

分 类 号：R599[医药卫生—内科学]