微型生化分析系统实验测试  

Experimental Testing of Micro Biochemical Analytical System

作　　者：陈刚[1] 温志渝[1] 温中泉[1] 徐溢[2] 李霞[1] 蒋子平[1] 

机构地区：[1]重庆大学光电工程学院,重庆400044 [2]重庆大学化工学院,重庆400044

出　　处：《光谱学与光谱分析》2005年第3期439-443,共5页Spectroscopy and Spectral Analysis

基　　金：国家"8 63"项目 (2 0 0 2AA40 4 0 2 3) ;国家自然科学基金重点项目 (0 94760 2 3);重庆市"十五"攻关项目 (7341 )资助

摘　　要：介绍了一种基于微型光纤光谱仪的微型生化分析系统及其相应的标定和实验测试。实验中对总蛋白、白蛋白、双氯芬酸钠、亚硝酸盐、碘 淀粉、Fe2 + 邻二氮菲等溶液体系的吸收谱进行了测试。根据不同浓度Fe2 + 邻二氮菲溶液体系的实验结果 ,作出了相应的工作曲线 ,结果表明该工作曲线具有很好的线性度。实验结果与日本岛津分光光度计、美国海洋光学公司的HR2 0 0 0微型光谱仪以及国产 72 2型分光光度计的实验结果进行了比较。实验测试表明该微型生化分析系统已达到实用化的要求 ,且具有实时全光谱 (36 0～80 0nm)分析、体积小等优点 ,是一种比较理想的微型生化分析系统。A micro biochemical analytical system based on a micro fiber spectrometer is introduced. Experiment was carried out to calibrate and test the analysis system. In the experiment, the absorption spectra of Fe2+-ferroin solution bodies with different concentrations were obtained. The working curve shows a fine linearity of the analysis system. The authors also compared the experimental results obtained from 722-spectrometer and those from our analysis system. It was shown that their system can meet the requirement of practical use. This system also has many advantages, such as real-time whole spectrum analyzing and small volume, and is an ideal instrument for biochemical analysis.

关 键 词：生化分析 双氯芬酸钠 白蛋白 实验结果 总蛋白 亚硝酸盐 国产 溶液体系 邻二氮菲 吸收谱 

分 类 号：O657[理学—分析化学] R446[理学—化学]