壳聚糖固定化脲酶活性的X射线微区分析  

X-Ray Microanalysis of the Activity of Immobilized Urease on Chitosan Membrane

作　　者：马晓莉[1] 姚子华[1] 

机构地区：[1]河北大学化学与环境科学学院

出　　处：《光谱学与光谱分析》2005年第3期456-459,共4页Spectroscopy and Spectral Analysis

基　　金：河北省自然科学基金 (1 960 61 )资助项目

摘　　要：利用X射线微区分析方法 ,对壳聚糖固定化脲酶活性进行了定位分析。筛选出了能捕捉固定化脲酶活性部位的捕捉剂BaCl2 ,以尿素作为底物 ,在Tris HCl缓冲溶液中 ,底物经固定化脲酶催化产生NH3 和CO2 ,后者和捕捉剂反应生成BaCO3 沉淀 ,沉积在固定化脲酶的催化活性部位 ,借此确定其催化活性部位。结果表明BaCl2 可用做固定化脲酶活性定位的捕捉剂。同时得到了壳聚糖固定化脲酶活性定位的最佳实验条件。The localization of the activity of immobilized urease on chitosan membrane was studied by X-ray microanalysis. BaCl(2) and urea were selected as the capture and substrate respectively. The substrate was hydrolyzed by immobilized urease to produce NH(3) and CO(2) in Tris-HCl buffer(pH 7.0), and the latter was captured by BaCl(2) to form precipitate. The precipite was deposited on the active site of immobilized urease. It is shown that the method is practicable and reliable. The optimum condition for the localization of activity of immobilized urease was studied.

关 键 词：脲酶活性 捕捉 部位 壳聚糖 利用 筛选 尿素 X射线微区分析 底物 固定化 

分 类 号：O643[理学—物理化学] R852.22[理学—化学]