汉族人群谷胱甘肽-S-转移酶M1、T1基因多态性分析  

The genetic polymorphisms of glutathione S-transterases.

作　　者：武守山[1] 邢朝斌[1] 

机构地区：[1]华北煤炭医学院生物科学系,唐山063000

出　　处：《中国优生与遗传杂志》2005年第3期15-16,共2页Chinese Journal of Birth Health & Heredity

基　　金：河北省科技厅科技攻关项目资助 (0 2 2 76110 6)

摘　　要：目的　分析汉族人群谷胱甘肽 -S -转移酶M 1、T1 (GSTM1、GSTT1 )的基因多态性分布。方法　样本为6 0名唐山地区汉族人群 ,采用多重等位基因聚合酶链反应 (PCR)方法分析GSTM 1和GSTT1基因多态性。结果　GSTM1缺失型和GSTT1缺失基因型频率分别为 33 3%和 1 1 7% ,同时具有GSTM1缺失型和GSTT1缺失型的个体频率为 1 7%。结论　唐山地区GSTM 1、GSTT1基因呈多态性分布 ,其等位基因和基因型频率不同于其他种族。Objective:In this paper,the distribution of gluta-thione S-transferases(GSTs) gene polymorphism in Chinese North Han people was reported. Methods:Genomic DNA was extracted from peripheral blood and the genotypes of GSTM1 and GSTT1 gene polymorphisms were analyzed by multiplex allele-specific polymerase chain reaction(PCR) in 60 Tang-shan Han people.Results:In this study population,The frequencies of GSTM1 null and GSTT1 null genotypes were 33.3% and 11.7% respectively and the frequency of the individuals with combination of GSTM1 null and GSTT1 null genotype was 1.7%. Conclusion:Distribution of the GSTM1,GSTT1 genes are polymorphic in Tangshan Han people and different from those in Caucasian and Black population.

关 键 词：GSTM1 GSTT1 谷胱甘肽-S-转移酶M1 基因多态性分布 汉族人群 缺失基因 合酶 PCR) 基因聚合 等位基因 

分 类 号：R346[医药卫生—基础医学]