高效液相色谱-紫外法测定指血中苯丙氨酸与酪氨酸浓度  

Determination of phenylalanine and tyrosine in finger blood by high-performance liquid chromatography with untraviolet detection.

作　　者：莫喜明[1] 唐爱国[1] 

机构地区：[1]中南大学湘雅第二医院检验科,湖南长沙410011

出　　处：《中国优生与遗传杂志》2005年第3期29-31,共3页Chinese Journal of Birth Health & Heredity

基　　金：湖南省科技厅资助项目 (0 4SK3 0 5 2 )

摘　　要：目的　探讨采用高效液相色谱法测定微量指血中苯丙氨酸和酪氨酸的可行性。方法　指血标本经去蛋白后取上清 ,用反相高效液相色谱 -紫外法进行检测。结果　苯丙氨酸线性范围为 6～ 1 5 1 2 μmol/L ,最低检测浓度为1 5 μmol/L ;平均回收率为 98 2 % ,批内CV为 3 2 % ,批间为 5 4 %。酪氨酸的线性范围为 5 5～ 1 2 5 0 μmol/L。最低检测限为 1 0 μmol/L ,平均回收率为 98 8% ,批内CV为 2 6 % ,批间为 4 6 %。 1 0 2例健康儿童指血苯丙氨酸浓度为 (6 7 76 4～ 1 5 4 3) μmol/L ,酪氨酸浓度为 (6 2 2 9± 1 3 97) μmol/L ,Phe/Tyr比值为 (1 1 5 4～ 0 .2 7)。结论　本法灵敏度好 ,特异性高 ,方法简便快速 ,适合于指血中Phe和Tyr的同时测定。Objective: To investigate the feasibility of determination of phenylalanine and tyrosine in finger blood by reverse phase high-performance liquid chromatography(RP-HPLC) method. Methods: Supernatant fluid of finger blood precipitated with perchloric acid was isocratically eluted using a base-deactivated C 8 column with 5% acetonitrile in water as the mobil phase. Untraviolet detector worked at 210nm. Results: Good linearity was observed in the range of 6～1512 μmol/L for phenylalanine, the lower limit of detection was 1.5μmol/L, the average recovery was 98.2%, and the intra-and inter-assay coefficients of variation (CV) were 3.2% and 5.4% respectively. There also was a good linearity in the range of 5.5～1250μmol/L for tyrosine. The average recovery was 98.8%, the intra-and inter-assay coefficients of variation (CV) were 2.6% and 4.6% respectively. The lower limit of detection was 1.0μmol/L.Phenylalanine and tyrosine in finger blood of 102 health enfant were (67.76±15.43)μmol/L and (62.29±13.97)μmol/L respectively. The ratio of phe/tyr was 1.15±0.27.Conclusion:This method is sensitivity, accurate, rapid and simple in measuring phenylalanine and tyrosine in finger blood.

关 键 词：指血 苯丙氨酸 酪氨酸 紫外法 高效液相色谱 健康儿童 血标本 平均回收率 酸浓度 微量 

分 类 号：R446.1[医药卫生—诊断学]