簇毛麦抗白粉病基因的RAPD及RFLP标记  

Characterization of RAPD Markers and RFLP Marker Linked to Powdery Mildew Resistant Gene Derived from Different H. villosa

作　　者：李辉[1,2,3] 陈孝[1] 施爱农[1] 孔凡晶[1] Leath S Murphy J P 贾旭[3] 

机构地区：[1]中国农业科学院作物育种与栽培研究所,北京100081 [2]河北省农林科学院粮油作物研究所,河北省遗传育种重点实验室,raleighnc27695 [3]中国科学院遗传与发育生物学研究所,国家植物细胞与染色体工程重点实验室,北京100101 [4]美国北卡罗来纳州立大学

出　　处：《中国农业科学》2005年第3期439-445,共7页Scientia Agricultura Sinica

基　　金：国家"九五"攻关项目资助(85-002-02-03);河北省自然科学基金资助项目(301450)

摘　　要：分析了来自前苏联簇毛麦及其抗病衍生系的抗白粉病基因对不同生理小种的抗性反应。用 120 个随机引物对 6D/6V 代换系 Pm930640 进行 RAPD 分析,检测到 5 个引物 OPAN03、OPAI01、OPAL03、OPAD07 和 OPAG15,分别在大约 1 700、700、750、480 和 580 bp 处有区别于小麦亲本的多态性条带。对 Chancellor×Pm930640 F2群体进行 OPAN03、OPAI01 和 OPAL03 等 3 个 RAPD 标记与抗白粉病基因的连锁分析,表明这些标记同簇毛麦的抗白粉病基因是连锁的。对大部分分别含有 Pm1-Pm20 的已知抗病基因、含有簇毛麦抗病基因及其相关亲本的 29 个小麦品系进行RAPD标记分析。结果表明,这些标记不仅可以鉴定簇毛麦的抗病基因,而且可以判断其遗传背景。OPAL03750仅出现在含有前苏联簇毛麦 6VS 染色体的抗病材料中,可作为区别于 Pm21 的分子标记。RFLP 标记的结果也表明两个不同簇毛麦的 6VS 染色体有明显的多态性。The analysis was carried out on performance of the resistant gene from H. villosa accession of the former Soviet Union to different isolates of Bluemerie graminis. Polymorphisms were revealed between 6D/6V substitution line Pm930640 and its pedigree parents using five RAPD markers of OPAN031700, OPAI01700, OPAL03750, OPAD07480 and OPAG15580 screened out from 120 random 10-mers primers. Three RAPD markers of OPAN03, OPAI01 and OPAL03 were linked with the resistant gene by analysis of F2 population of Chancellon×Pm930640. Analysis of RAPD markers of 29 wheat lines, including lines conferring one of the most genes from Pm1 to Pm20 respectively, lines conferring resistant gene from two H. villosa accessions and the related wheat parents, showed that these markers not only linked to the gene resistant to powdery mildew from H. villosa, but also detected different genetic backgrounds. OPAL03750 can be used as the marker to distinguish the different resistant lines from two H. villosa accessions because it was only observed in the materials from H. villosa of the former Soviet Union. RFLP analysis also showed the polymorphisms between two H. villosa accessions and their derived resistant lines.

关 键 词：簇毛麦 抗白粉病基因 RFLP标记 抗病基因 小麦 亲本 RAPD F2群体 染色体 随机引物 

分 类 号：S512.1[农业科学—作物学]