新型基因工程亚单位菌苗对猪传染性胸膜肺炎的保护效力研究  

Study on the Protective Efficiency of the Genetic Subunit Bacterin Against Porcine Contagious Pleuropneumonia

作　　者：刘建杰[1] 陈焕春[1] 李冲[1] 何启盖[1] 吴斌[1] 

机构地区：[1]华中农业大学动物医学院动物病毒室,武汉430070

出　　处：《中国农业科学》2005年第3期596-600,共5页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目(30200011);湖北省重点科技攻关项目资助(2001AA201B02)

摘　　要：利用分泌毒素 ApxⅠ、ApxⅡ、ApxⅢ的重组表达产物和胸膜肺炎放线杆菌 7 型菌,研制了一种新型的基因工程亚单位菌苗并对此疫苗的免疫效力进行了研究。利用该疫苗免疫断奶仔猪,间隔 3 周加强 1 次,同时设空白对照组和一种商品化的三价细菌灭活苗组。首免后每周采血检测 ApxⅠ、ApxⅡ、ApxⅢ的 ELISA 抗体和 7 型菌的血凝抗体,第 2 次免疫两周后用猪胸膜肺炎放线杆菌 1 型菌株进行气管攻毒。从攻毒后症状、细菌分离、抗体检测、死后剖检对免疫效果进行评价。结果显示亚单位菌苗对于猪传染性胸膜肺炎有显著的保护力,能明显减轻临床症状和降低肺部损伤,效果优于商品化的细菌灭活苗。The efficacy of a new genetic subunit bacterin containing recombinant Apx toxins and serovar 7 of Actinobacillus pleuropneumoniae was determined. Weaned piglets were vaccinated twice intramuscularly with the new genetic subunit bacterin or commerical trivalent formalin-inactivated A. pleuropneumoniae. The antibody of each antigen was detected each week after first immunization. Piglets were challenged by percutaneous intratracheal injection with serovar 1. The protective efficacy of immunization was evaluated by clinical, bacteriological, serological and post-mortem examination. The results suggest that immunization with subunit bacterin could reduce clinical sign and lung lesion, which is more efficacious than bacterins.

关 键 词：猪传染性胸膜肺炎 菌苗 灭活苗 ELISA抗体 猪胸膜肺炎放线杆菌 断奶仔猪 亚单位 保护效力 细菌 气管 

分 类 号：S858.28[农业科学—临床兽医学]