山羊(Bore)-兔异种克隆胚胎连续核移植的研究  

Study of Serial Nuclear Transfer on Goat (Bore)-Rabbit Inter-Species Cloned Embryo

作　　者：章志国[1] 章孝荣[1] 刘亚[1] 金仁桃 章美玲[1] 汪存利[1] 赵焕[1] 

机构地区：[1]安徽农业大学动物科技学院 [2]安徽省应医院,合肥230022

出　　处：《中国农业科学》2005年第3期601-605,共5页Scientia Agricultura Sinica

基　　金：安徽省"十五"重大科技专项资助项目(01603006)

摘　　要：以山羊-兔异种克隆桑椹胚卵裂球为核供体,兔卵母细胞为受体进行连续核移植研究,最终连续 2 次,共获得继Ⅰ代重构胚 58 枚,继Ⅱ代重构胚 14 枚。融合率分别为 79.5%和 70%,差异不显著(P>0.05)卵裂率分别为 75.9%和 28.6%,差异显著(P<0.05)。但继Ⅱ代重构胚最终未能发育至囊胚,继Ⅰ代重构胚囊胚率达到 10.2%。The experiments of serial nuclear transfer were conducted by using the blastomere of inter-species original or serial cloned morula as donor and rabbit oocyte as recipient. Finally serial I and serial II cloned embryos were obtained. Of these, Ⅰserial reconstructed embryos 58 andⅡserial reconstructed embryos 14 .The rate of fusion was 79.5% and 70% respectively and there was no significant difference between them(P>0.05). However, the rate of cleavage respectively was 75.9% and 28.6% and difference between them was very significant (P<0.05). Eventually there was noⅡserial blastocyst obtained, but the rate of Ⅰserial blastocyst was 10.2%.

关 键 词：山羊 异种克隆 囊胚率 桑椹胚 核移植 胚囊 卵裂率 重构胚 胚胎 卵裂球 

分 类 号：S814.8[农业科学—畜牧学]