中药“参阳”方冲剂对舌鳞癌SD大鼠外周血淋巴细胞亚群的调节作用  

Modulation of Chinese regimen granules of Shenyang on peripheral blood lymphocyte subsets of SD rats with SCC of tongue

作　　者：蒋灿华[1] 叶冬霞[1] 陈万涛[1] 张秀丽[1] 张志愿[1] 邱蔚六[1] 

机构地区：[1]第二医科大学附属第九人民医院口腔医学院口腔颌面外科

出　　处：《中华口腔医学杂志》2005年第2期118-121,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金 ( 30171014;30300388;30330580 );上海市科学技术发展基金 ( 014319359;036505013;034107002)资助项目

摘　　要：目的　在临床前瞻性研究已表明中药“参阳”方冲剂能够延长口腔鳞癌术后患者的生存期并提高生存率的基础上,观察“参阳”方冲剂对舌鳞癌SD大鼠外周血淋巴细胞亚群的影响并探讨其可能的免疫学机制。方法　80只SD大鼠采用 0 002 % 4 硝基喹啉 1 氧化物自然饮水喂养 36周,诱发建立舌鳞癌动物模型共 61只,随机分为 4组:“参阳”方冲剂高剂量组 16只,“参阳”方冲剂正常剂量组、阳性对照(刺五加)组和空白对照组各 15只。药物干预 15天,分别于给药前 1天、给药结束后 1天采集外周静脉血,双色荧光抗体标记流式细胞术分析淋巴细胞亚群的变化,自身前后对照配对t检验进行统计学分析。结果　“参阳”方冲剂能增加外周血CD3+CD4+T淋巴细胞与CD3-CD161a+NK细胞百分比,提高CD4+ /CD8+比值,降低CD3+CD8 +T淋巴细胞百分比。对外周血CD3-CD161a+NK细胞百分比提高方面,其效果优于刺五加。结论　纠正机体T淋巴细胞亚群的紊乱并提高CD3-CD161a+NK细胞比例,可能是“参阳”方冲剂发挥抗癌作用的主要效应机制之一。Objective Prospective research demonstrated that Chinese regimen granules of Shenyang could prolong survival time and improve survival rate of patients with oral squamous cell carcinoma (SCC). But the mechanism was not clear. The purpose of this study was to investigate Shenyang′s effect on peripheral blood lymphocyte subsets of SD rats with SCC of tongue and explore immunological mechanism. Methods Among 80 SD rats fed by 0 002% 4 nitroquinoline 1 oxide (4NQO) drinking water for 36 weeks, 61 rats with SCC of tongue had been found and were randomly divided into 4 groups, namely Shenyang A, Shenyang B, positive and blank control groups. Before and after high and normal dosage of Shenyang , acanthopanax senticoside and water had been given for 15 days respectively, peripheral blood lymphocyte subsets were detected with flow cytometry. The data were statistically analyzed with paired t Test. Results Percentage of CD3+ CD4+ T cell and CD3-CD161a+NK cell, ratio of CD4+/CD8+ were increased. Percentage of CD3+CD8+ T cell was decreased, and the effect was better than that of acanthopanax senticoside in improving the percentage of CD3-CD161a+ NK cell.Conclusion Among anti tumor mechanisms of Shenyang it is that corrects disorder of lymphocyte subsets and increases percentage of CD3-CD161a+ NK cell.

关 键 词：中药 “参阳”方冲剂 舌鳞癌 SD大鼠 外周血 淋巴细胞亚群 调节作用 

分 类 号：R285[医药卫生—中药学]