大豆疫霉根腐病菌毒素的提取及生物活性测定  

Study on Extraction and Bioactivity Assay of Pathotoxin Produced by Phytophthora sojae

作　　者：张淑珍[1] 徐鹏飞[1] 武小霞[1] 赵孝武[1] 张大勇[1] 姜振峰[1] 李文滨[1] 

机构地区：[1]东北农业大学大豆研究所,哈尔滨150030

出　　处：《中国农学通报》2005年第3期252-254,315,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金(30400285)"大豆疫霉根腐病的分离纯化;生理小种鉴定;抗源筛选及致病过程中基因表达序列标签分析"项目资助。

摘　　要：对大豆疫霉根腐病原菌的主要致病因子之一—毒素的提取方法进行了探讨,并用抗感不同大豆品种的切根苗进行了毒素生物活性及最佳浓度的测定、筛选。结果表明:毒素的提取采取弱极性的有机溶剂萃取的方法比较理想,尤其是用乙醚萃取;用稀释100×浓度的毒素处理抗感不同的大豆品种的切根苗可以产生与病原菌下胚轴接种法相同的症状。In this paper, the extraction and bioactivity assay of pathotoxin produced by Phytophthora sojae (Pmg.) were systematically researched. The main results were as follows: The best extraction way of the pathotoxin produced by Pmg was to use the weak polarity organic solvent, especially ether. The rude crystalline of the pathotoxin was gotten by continuous extraction by ether; The bio-activity of the rude pathotoxin was determined by the seedlings with cutting roots, and the symptom of seedlings with cutting roots treated with the concentration of the dilute factor of 100 appeared the same as those with the hypocotyl inoculation. This also proved that the pathotoxin was a kind of Non- Host Specitiy Toxin.

关 键 词：大豆疫霉根腐病 切根苗 病菌毒素 生物活性测定 大豆品种 接种法 下胚轴 病原菌 致病因子 处理 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治] S791.248[农业科学—植物保护]