紫外线杀灭营养液中黄瓜枯萎病病菌试验研究  

UV Disinfection of Fusarium oxysporum f.sp.cucumerinum in Drain Water from Closed System

作　　者：刘向辉[1] 刘芬[1] 周立刚[2] 宋卫堂[3] 

机构地区：[1]中国农业大学工学院,北京100083 [2]中国农业大学农学与生物技术学院,北京100094 [3]中国农业大学理学院,北京100094

出　　处：《中国农学通报》2005年第3期268-271,共4页Chinese Agricultural Science Bulletin

基　　金：农业部"948"项目(201072)。

摘　　要：设计、试制了一种可以用于无土栽培循环营养液消毒的紫外线消毒系统,并对其杀灭黄瓜枯萎病病原菌的性能和效果进行了实验室研究。先将黄瓜枯萎病病原(Fusariumoxysporumf.sp.cuc鄄umerinum)菌株接种于PDA培养基上,培养后将其加入到营养液中进行紫外线照射。通过对紫外线照射前后的病原浓度进行计数,发现紫外线对黄瓜枯萎病病原有较好的杀灭效果。在营养液紫外线透射率(T10)为96.65%、病原浓度为6.87×102conidia/ml、最大流量为36L/min时,灭菌率为100%;在营养液紫外线透射率(T10)为95.42%、病原浓度为1.14×105conidia/ml、最大流量为36L/min时,灭菌率为100%;在T10降低为11.58%、病原浓度为1.44×104conidia/ml、最大流速为35L/min时,灭菌率也在50%以上。In this paper, an installation of UV disinfection was designed, and its efficacy against the Fusarium oxysporum f.sp.cucumerinum which is a kind of root-infecting pathogens, was tested in lab. The cultures of Fusarium oxysporum f.sp.cucumerinum were grown in potato dextrose and introduced artificially to a tank that contained enough water. By examining the number of spores grown in the plate of potato dextrose agar before and after being disinfected, it was found that Ultra-violet radiation is effective against Fusarium oxysporum f.sp.cucumerinum The pathogens were totally killed when the water(T10=96.65%) flowed at 36L/min and the pathogen concentration was 6.87×102conidia/ml. Even if the concentration of pathogen was 1.14×105conidia/ml, the spores could not survive when the water(T10=95.42%) flowed at 36L/min. And when the water(T10=11.58%) flowed at 7.9 L/min and 35L/min, the disinfection rate was 81.31% and 50%, respectively.

关 键 词：营养液 黄瓜枯萎病 病原 浓度 PDA培养基 病菌 无土栽培 紫外线 灭菌 杀灭 

分 类 号：S436.421.1[农业科学—农业昆虫与害虫防治] S646[农业科学—植物保护]