霍乱弧菌O139感染棘阿米巴的实验研究  

Survival and growth of Vibrio cholerae O139 inside Acanthamoeba

作　　者：姜庆五[1] 李勤学[1] 陈政[2] 沈洁[1] 钟培松[2] 张欣[2] 谈佳弟[2] 邵月琴[2] 李子华[1] 

机构地区：[1]复旦大学公共卫生学院,200032 [2]上海市嘉定区疾病预防控制中心

出　　处：《中华流行病学杂志》2005年第3期204-206,共3页Chinese Journal of Epidemiology

摘　　要：目的　探讨霍乱弧菌能否在阿米巴原虫内生存。方法　采用霍乱弧菌O139 与多噬棘阿米巴的共培养方法,通过倒置显微镜、革兰染色和电镜超薄切片观察霍乱弧菌在阿米巴滋养体和包囊内生存情况。结果　培养24 h,革兰染色可见霍乱弧菌O139 已进入阿米巴滋养体的吞噬泡内,随共培养时间的延长,可见吞噬泡内霍乱弧菌O139增多。电镜下可见霍乱弧菌O139 在阿米巴环境中的不同感染期:包括胞饮期、吞噬泡形成期、增殖期及滋养体裂解释放,部分感染的滋养体发生包囊化,胞浆吞噬泡内可见存活O139弧菌。结论　霍乱弧菌 O139 能在棘阿米巴滋养体内生存繁殖,并存活于包囊胞浆的吞噬泡中,因此棘阿米巴原虫可能是霍乱弧菌O139的环境贮存宿主之一。Objective To study the survival and growth of Vibrio cholerae inside the Acanthamoeba polyphage. Methods Survival and growth of Vibro cholerae O139, co-cultured with Acanthamoeba polyphaga, was observed inside the trophozoites and cysts, using Gram stain and electron microscope. Results Viable O139 was observed inside the amoebal vacuoles in 24 hours. Vacuoles were filled with more bacteria along with the longer period of co-culture. The process of O139 infection with Amoebae would include uptake, formation of O139 vacuole, multiplication, trophozoites lysed and expel under electron microscopy. Some infected trophozoites could subsequently encyst and the surviving O139 could locate in the vesicles inside the cysts. Conclusion O139 might survive and multiply in the trophozoites and reside inside the cysts of Amoebae, suggesting that Acanthamoebae might serve as one of the environmental hosts of Vibro cholerae.

关 键 词：霍乱弧菌 吞噬 棘阿米巴 滋养体 感染 革兰染色 胞浆 繁殖 存活 原虫 

分 类 号：Q93-3[生物学—微生物学]