RFLP分子标记及其在蔬菜研究中的应用  

RFLP Molecular Marker and Its Application to Vegetable Researches

作　　者：邓俭英[1] 刘忠[1] 康德贤[1] 张玲玲[2] 王绪[2] 方锋学[1] 

机构地区：[1]广西农业科学院蔬菜研究中心,南宁530007 [2]广西大学农学院,南宁530005

出　　处：《分子植物育种》2005年第2期245-248,共4页Molecular Plant Breeding

摘　　要：RFLP技术是一种高效的基因组DNA多态性分析技术,其原理是用限制性内切酶切割不同个体基因组DNA后,检测含同源序列的酶切片段在长度上的差异,具有可靠性较高,但操作烦琐、信息含量低等特点。本文综述了RFLP分子标记技术在蔬菜分类学及遗传多样性研究、遗传图谱构建、基因定位、分子标记辅助育种方面的应用情况,并对其应用前景进行了展望。Restriction fragment length polymorphism, that is briefly called RFLP, is a high-efficiency technology for the polymorphism of genome DNA analysis. RFLP was that deavage individuals DNA with restriction enzyme could make different length fragments of resistance gene aralogs, with more reliability, complicated manipulation and lower information. This paper reviewed the recent advance of the application of RFLP in various aspects of vegetable researches including genetic map construction, gene location and markers assisted selection.

关 键 词：蔬菜 分子标记辅助育种 RFLP技术 酶切 遗传图谱构建 分子标记技术 遗传多样性 限制性内切酶 切片 研究 

分 类 号：S63[农业科学—蔬菜学]