用闭合三角形杂交群体检测QTL等位位点差异  

Detection of Allelic Diversity of QTLs with Closed Triangle Cross Populations

作　　者：廖艳[1] 胡轶[1] 李晚忱[1] 

机构地区：[1]四川农业大学玉米研究所,雅安625014

出　　处：《分子植物育种》2005年第2期269-274,共6页Molecular Plant Breeding

摘　　要：本文简述QTL定位和数量性状分子标记辅助选择的基本原理、基本方法,以及在实际应用中存在的一些问题。将12个不同作图群体构建的RFLP或SSR分子标记连锁图,根据其大多数分子标记所在的染色体节,再按每条染色体在各自连锁图中的相对长度整合在一起,然后将所定位的玉米株高QTL转换成整合图上的相对位置。结果发现,12个作图群体定位的75个玉米株高QTL位点遍布整个染色体组,QTL定位的重演性不高。分析认为,这是数量性状受众多微效多基因控制的必然现象。最后,提出用闭合三角形杂交群体进行QTL定位,检测QTL等位点的多重差异,并设想用这种方法验证或提高不同作图群体定位同一数量性状QTL的重演性。A summary was made on basic principle and method of QTL location and molecular marker assisted selection for quantitative traits, as well as some problems in application. According to the chromosome bins including most of the molecular markers and the comparative length of each chromosome in its own linkage map, 12 RFLP or SSR molecular marker linkage maps constructed with different mapping populations were integrated into one, and the identified QTLs of maize plant height were turned to comparative locations on the integrated map. The result showed that the 75 identified QTLs of maize plant height distributed all over the chromosome set and the recapitulation of QTL location was low. This phenomenon was thought to be due to multiple genes controlling a quantitative trait. At the end, it was proposed to locate QTLs with closed triangle cross populations and detect allelic diversity of QTLs. By this method, it was attempted to examine or raise the recapitulation of QTL location of the same quantitative trait with different mapping populations.

关 键 词：QTL定位 杂交群体 位点 作图群体 数量性状 株高 玉米 体检 闭合 染色体 

分 类 号：S336[农业科学—作物遗传育种]