旱作转BADH基因水稻旗叶、不定根解剖结构的研究  

Anatomical Structure on Flag Leaves and Adventitious Root of Transgenic Rice with BADH cDNA in Rainfed Farming

作　　者：郭学民[1] 东方阳[1] 孙耀中[1] 刘永军[1] 杨晓玲[1] 高红[1] 

机构地区：[1]河北科技师范学院生命科学系,河北昌黎066600

出　　处：《西北植物学报》2005年第3期454-459,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：秦皇岛市科技局资助项目

摘　　要：用石蜡切片法比较研究了 6个水稻品系 品种 5 2 - 7、5 1- 15、5 1- 2 2、中 8、开系 7、白珍珠的旗叶、上层不定根的显微结构 .结果表明 ,转 BADH基因水稻品系 品种 旗叶叶片及其角质层比中 8、开系 7相对较厚 ,叶肉细胞层数及主脉维管束数比中 8、开系 7的多 .转 BADH基因水稻品系叶鞘的维管束数、气腔数目比中 8、开系 7、白珍珠多 ,其外表皮角质层比中 8、开系 7、白珍珠相对较厚 ,其维管束面积与中 8、开系 7、白珍珠相差不大 .除 5 1- 15外 ,6个水稻品系 品种 上层不定根的大导管数基本相同 ,转 BADH基因水稻品系的小导管数较多 ,大、小导管的面积和以及导管的总面积和均大于中 8和白珍珠 ,皮层比中 8、开系 7、白珍珠相对较薄 .转 BADH基因水稻品系的上述结构特征说明其旗叶生产的光合产物可满足穗部籽粒充实的需要 ,上层不定根发达的维管组织有利于根部水、无机盐的向上运输 ,提高物质运输量 ,为高产奠定了物质基础 .The ultratructure of the flag leaves and upper adventitious roots for six rice strains (cultivars) were studied by paraffin method.The results showed that the flag leaves and their cuticle of transgenic rice with BADH cDNA strains were thicker than those of Zhong 8 and Kaixi 7.The numbers of mesophyll cell and midrib vascular bundle were also more than those of Zhong 8 and Kaixi 7.The numbers of vascular bundle and air chamber for leave-sheath of transgenic rice with BADH cDNA were more than those of Zhong 8,Kaixi 7 and white pearl.The area of vascular bundle was not obviously different from Zhong 8,Kaixi 7 and white pearl.The number of upper adventitious root big vessel of all rice used in the experiment is similar except 51-15,but the number of small vessel for transgenic rice with BADH cDNA was much more than the others'.The total area of vessel was bigger than those of Zhong 8 and white pearl.Their Cortex was thinner than those of Zhong 8,Kaixi 7 and white pearl.These results indicateed that the photosynthate of flag leaves of transgenic rice with BADH cDNA could meet the need of seeds for filling,and the advanced vascular tissue of upper adventitious roots is beneficial for the antrorse transport of water and mineral salt in roots,and those might lay the material foundation for high field.

关 键 词：转BADH基因水稻 旗叶 叶鞘 上层不定根 

分 类 号：Q944.54[生物学—植物学] S511[农业科学—作物学]