人类新型β3半乳糖基转移酶基因β3GalT7的克隆和鉴定  

Cloning and Characterization of a Novel Human β3-Galactosyltransferase Gene β3GALT7

作　　者：周嘉梁[1] 黄超群 吴士良[2] 孙其昌 雷伟 金美芳 姜智 沈宏杰 

机构地区：[1]苏州大学医学院基础医学系生物化学与分子生物学教研室,苏州215007 [2]苏州大学生化工程研究所,苏州215007

出　　处：《生物化学与生物物理进展》2005年第2期140-146,共7页Progress In Biochemistry and Biophysics

基　　金：苏州大学医学发展基金资助项目.~~

摘　　要：从人肺cDNA文库中克隆到人类β3-半乳糖基转移酶家族中的一个新成员β3GalT7(AY277592,EC2.4.1.-),并对其进行了鉴定. 该基因定位在人类染色体19q13.2. 它包含一个1 191 bp的开放阅读框(ORF). 编码的蛋白质包括一个信号肽和一个半乳糖基转移酶结构域,分子质量和等电点分别为43.3 ku和8.37. 从原核表达中获得的融合蛋白的分子质量和预测相符. RNA印迹杂交显示β3GalT7在肺、喉和回肠组织中高表达,而在舌、乳房、子宫、睾丸等组织中表达较低. 此外半定量RT-PCR显示β3GalT7在人类不同的肿瘤细胞中转录水平有明显差异.A novel human β3-galactosyltransferase gene β3GALT7 was isolated from human lung cDNA library and characterized. β3GALT7 is mapped to chromosome 19q13.2 by browsing the UCSC genomic database. It contains an ORF with length of 1 191 bp, encoding a protein with a signal peptide sequence and galactosyl-T domain, and its molecular mass and isoelectric point are predicted to be 43.3 ku and 8.37 respectively. The molecular mass of recombinant β3GALT7 protein expressed in prokaryote was coincide with the predicted. Northern blot showed that β3GALT7 was highly expressed in lung, throat and ileum, while the expression level was low in tongue, breast, uteri, testis. In addition, it was also demonstrated that β3GALT7 is differentially transcribed in human tumor cell lines.

关 键 词：β3GalT7 克隆 RNA印迹 肿瘤细胞株 

分 类 号：Q53[生物学—生物化学]