猪流产鹦鹉衣原体的抗原结构及抗原性和免疫原性分析  

ANTIGENIC STRUCTURE AND ANTIGEENIC AND IMMUNOGENIC ANALYSIS OF CHLAMYDIA PSITTACI ISOLATED ROM ANORTED PIGS

作　　者：段跃进[1] 杨学军[1] 李英才[1] 帅永玉[1] 高双娣[1] 杨学礼[1] 

机构地区：[1]中国农科院兰州兽医研究所

出　　处：《畜牧兽医学报》1994年第6期551-554,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：农业部"八五"攻关重点项目;国家自然科学基金

摘　　要：本试验用ＳＤＳ－ＰＡＧＥ技术对分离自青海、湖北、广西省流产猪的鹦鹉衣原体抗原结构进行分析，发现这些菌株的抗原结构图谱相似，即共同大有主外膜蛋白ＭＯＭＰ和其他一些主多肽。并以Ｄ１３株免疫兔子制备的抗血清作为探针，用免疫印迹试验检查其与各株鹦鹉衣原体抗原分子的反应关系。证实Ｄ１３株的主要抗原３８ＫＤ（ＭＯＭＰ）、６０ＫＤ、８０ＫＤ和１１０ＫＤ具有抗原性，它们可刺激机体产生抗体免疫应答。该抗血清不仅可与本株的抗原分子发生反应，也与其他猪流产鹦鹉衣原体的３８ＫＤ、６０ＫＤ、８０ＫＤ和１１０ＫＤ蛋白质抗原反应。说明本试验所检查的鹦鹉衣原体具有相同的抗原结构和相同的抗原性和免疫学反应特性。In this report we analysed the antigenic profile of Chlalnydia psittaci isolated fromryaborted pigs in Qinghai,Hubei and Guangxi by using SDS-PAGE technique,in whichthe MOMPs and other major polypeptides had been demonstrated in all tested chlamydialstrains and no obvious difference of antigenic structure was observed among thesebacterial strains.By immunizing healthy rabbit with D13 strain, we produced antiserumfor western blotting for antigenicity and immunogenicity analysis.In western blotting,the antiserum reacted with 38KD 60KD,80KD and ll0KD proteins of all chlamydialotrains,which showed that these C. psittaci strains share the same antigenic and immuno-genic reaction characteristics.

关 键 词：鹦鹉衣原体 免疫原性 抗原 结构 猪病 流产 

分 类 号：S858.287.2[农业科学—临床兽医学]