共表达红光荧光蛋白及发夹RNA载体的构建及鉴定  

Construction and identification of vectors encoding both red fluorescent protein and shRNA

作　　者：魏映辉[1] 吴秀丽[1] 万敏[1] 于永利[2] 王丽颖[1] 

机构地区：[1]吉林大学基础医学院分子生物学教研室,吉林长春130021 [2]吉林大学基础医学院免疫学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2005年第2期163-165,共3页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金海外杰出青年基金项目资助课题 (30 32 80 10 )

摘　　要：目的 :构建共表达红光荧光蛋白及短发夹状 RNA的重组真核载体。方法 :利用亚克隆、T- A克隆和PCR技术构建 pc DNA3.0 / Ds Red- U6 - sh GFR及 pc DNA3.0 / Ds Red- U6重组载体。结果 :成功构建上述两种真核重组表达载体。结论 :这种共表达载体的构建为进一步利用 RNAi技术研究真核细胞基因功能奠定了基础。Objective To construct the recombinant vectors that coexpress red fluorescent protein and small hairpin RNA (shRNA). Methods The subcloning, T-A cloning, and PCR technique were used to construct the recombinant vectors,named pcDNA3.0/DsRed-U6-shGFP and pcDNA3.0/DsRed-U6. Results The recombinant expression vectors mentioned above were successfully constructed.Conclusion The successful construction of recombinant vectors can establish a feasibility to further explore the functions of specific genes in eukaryotic cells with RNAi technique

关 键 词：短发夹状RNA 发光蛋白质类 质粒 

分 类 号：Q78[生物学—分子生物学]