滋补脾阴方药联合牛脑提取液治疗大鼠脊髓损伤实验研究  

Experimental study of the compositive applying of nourishing Piyin Remedy and bovine brain extract on spinal cord injury of rats

作　　者：王鸿飞[1] 富灵杰[2] 战丽彬[1] 郑连杰[1] 张书琴[1] 刘用楫[1] 苗艳颖[3] 

机构地区：[1]大连医科大学第二附属医院骨科,辽宁大连市116023 [2]大连医科大学 [3]北京大学医学部,北京市100083

出　　处：《中国康复理论与实践》2005年第2期96-98,共3页Chinese Journal of Rehabilitation Theory and Practice

摘　　要：目的 探讨滋补脾阴方药与牛脑提取液对实验性大鼠脊髓损伤(SCI)的作用和机理。方法 健康 SD大鼠 80 只,随机分为硬膜下给牛脑提取液组、硬膜下给生理盐水组、滋补脾阴方药组、口服生理盐水组及联合给药组。采用改良 Allen氏装置制作动物模型。观察各组动物的BBB评分、体感诱发电位、辣根酶示踪、大体标本、组织学及超微结构变化。结果 硬膜下给牛脑提取液组、滋补脾阴方药组与各自对照组相比,各项指标皆有明显改善;联合给药组各项指标优于单独用药组。结论 滋补脾阴方药能阻止脊髓继发性损伤,促进SCI大鼠神经功能的恢复;牛脑提取液可促进SCI大鼠神经纤维的修复与再生;两者联合应用具有协同作用。ObjectiveTo study the effect and mechanisms of nourishing Piyin Remedy (nPR) and bovine brain extract (bBE) on experimental spinal cord injury (SCI) of rat.Methods80 healthy SD rats were divided into 5 equal groups randomly: bBE group supplied through subarachnoid cavity, normal saline (NS) group supplied through subarachnoid cavity, nPR group, NS orally taken group, combined group. Animal models were made by Allen's equipment on T 8～T 9 segment. The spinal nerve function, somatosensory evoked potentials (SEP), retrograde and label technique of horseradish peroxidase, gross observation, histological and morphometric analysis were taken as the observed indices.ResultsThe values of observed indices of bBE group and nPR group improved evidently compared with their own control groups; that of combined group was prior to sole administration.ConclusionnPR can hold back the secondary SCI and accelerate the recovery of spinal nerve function; bBE can stimulate the improvement of injuried nerve fibers; the joint of nPR and bBE can make a synergic effect. [

关 键 词：脊髓损伤(SCI) 滋补脾阴方药 牛脑提取液 大鼠 

分 类 号：R651.2[医药卫生—外科学] R255.6[医药卫生—临床医学]