Earle's液与MEM培养基对神经元活性的影响  

Effect of Earle's solution and MEM on activities of neurons in culture

作　　者：韩明[1] 董丽萍[1] 袁芳 

机构地区：[1]北京市神经外科研究所,北京市100050

出　　处：《中国康复理论与实践》2005年第2期125-126,共2页Chinese Journal of Rehabilitation Theory and Practice

基　　金：北京市科技新星计划项目(No. 953813000);北京市自然科学基金项目(No. 7043065)

摘　　要：目的 观察Earle's液、新配最小必需培养基(MEM)及原 MEM对神经元活性的影响。方法 神经元培养 10 d后,分别换为Earle's液、新配MEM及原MEM,12 h和 24 h后用乳酸脱氢酶(LDH)试剂盒测定细胞的 LDH漏出量,四甲基偶氮唑盐(MTT)微量酶反应比色法测定MTT代谢率。结果 神经元在不同培养介质中孵育 12 h和 24 h后,细胞 LDH的漏出量及 MTT代谢率有非常显著性差异( P <0.01)。结论 不同孵育液对神经元的活性有影响。ObjectiveTo observe the activities of cultured neurons incubated with Earle's solution, new minimal essential medium (MEM) or former MEM.MethodsActivity changes of cultured neurons were measured by determining the leakage of lactate dehydrogenase (LDH) and metabolic rate of 3 (4,5 dimethylthiazol)2,5 diphenyltetrazolium bromide (MTT).ResultsActivities of neurons incubated with different culture medium for 12 h or 24 h were significantly different ( P<0.01).ConclusionDifferent culture medium can influence neuronal activities. [

关 键 词：神经元 Earlés液 最小必需培养基(MEM) 乳酸脱氢酶 四甲基偶氮唑盐(MTT) 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]