猪Ⅱ型圆环病毒ORF2基因的克隆及其序列分析  

Cloning and Sequence Analysis of ORF2 Gene of Porcine circovirus Type 2

作　　者：蒋智勇[1] 宋长绪[1] 王贵平[1] 高向阳[2] 赵亚华[2] 

机构地区：[1]广东省农业科学院兽医研究所,广东广州410640 [2]华南农业大学生命科学院,广东广州410642

出　　处：《动物医学进展》2005年第3期63-66,共4页Progress In Veterinary Medicine

基　　金：广东省农业攻关项目;广州市农业攻关项目(2003B21404)

摘　　要：根据GenBank中猪圆环病毒Ⅱ型(PCV-2)ORF2基因序列,设计一对引物,应用PCR从疑似断奶仔猪多系统消耗综合征(PMWS)的死亡仔猪组织病料中扩增出ORF2基因(702 bp).将此基因片段克隆入pMD 18-T载体,筛选获得重组质粒pMD-ORF2并对其测序,结果表明所克隆的ORF2基因与德国分离株AF201897核苷酸序列同源性为99.5%.与其它PCV-2的ORF2核苷酸序列同源性在92.1%～99.9%之间,推导的氨基酸序列同源性在90.2%～99.5%之间.According to the published ORF2 genes sequence of PCV-2,a pair of primer was designed, and ORF2 genes was amplified from the suspected PMWS sample using PCR. Then the PCR products was cloned into pMD 18-T easy vector,and the recombinant plasmids named pMD-ORF2 were obtained. The sequences of the cloned PCR products was analyzed and compared with other PCV-2 isolates in GenBank.The results indicated that the ORF2 gene closely related with a German isolate AF 201897,the homology of their nucleotide sequence was 92.1% to 99.9%. The ORF2 gene displayed 93.4% to 99.7% identity based on amino acids with other PCV-2 strains.

关 键 词：ORF2 猪Ⅱ型圆环病毒 同源性 PCV 病料 多系统消耗综合征 猪圆环病毒 基因 克隆 核苷酸序列 

分 类 号：S852[农业科学—基础兽医学] S858[农业科学—兽医学]