NDV La Sota株、V4株F蛋白表位的预测  

Prediction of Antigenit Epitopes on F Proteins of NDV La Sota and V4

作　　者：孙建宏[1] 曹殿军[1] 刘培欣[1] 闫丽辉[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001

出　　处：《动物医学进展》2005年第3期72-75,共4页Progress In Veterinary Medicine

基　　金：国家高技术研究发展计划(863计划)项目(2003AA241110)

摘　　要：为进一步研究 2 种疫苗的表位,以深入探讨NDV La Sota株、V4疫苗免疫差异的根本所在。应用亲水性、可及性、抗原性、可塑性和二级结构预测等 5 种参数分别对新城疫病毒 La Sota株和 V4 株 F蛋白的 B细胞抗原表位进行了综合预测,预测到二者的 B细胞抗原表位分别有 19 个,这些表位中含有已经确定的 5 个中和表位, A1: 72aa, A2:78aa, A3: 79aa, A4: 157 aa、161 aa、170 aa、171 aa,A5:343 aa。比较发现,二者有 9 个预测的B细胞表位氨基酸序列存在差异。To study epitopes of NDV La Sota and V4 vaccines and reveal the differences of immunity of the two vaccines,B cell-epitopes on F protein of NDV La Sota and V4 strain were predicted by Hydrophilicity,Flexibility,Accessibility,Antigenicity and Secondary structure index. It was showed that there were 19 B cell-epitopes on F protein of NDV La Sota and V4 strain respectively, which include 5 confirmed neutral epitopes: 72aa,78aa,79aa,157aa,161aa,170aa,171aa,343aa and also with 9 B cell-epitopes confirmed.

关 键 词：抗原表位 B细胞表位 F蛋白 NDV 预测 抗原性 疫苗免疫 种参 新城疫病毒 氨基酸序列 

分 类 号：S852[农业科学—基础兽医学] R392[农业科学—兽医学]